血清玻连蛋白在口腔鳞癌中表达及临床意义研究  

Expression and Significance of Vitronectin in Blood Serum of Oral Squamous Cell Carcinoma

作　　者：田兵 胡腾龙[1] 静广平[1] 于洋[1] 钟克涛 王海涛 俞晴 

机构地区：[1]哈尔滨医科大学附属口腔医院,黑龙江哈尔滨150001

出　　处：《口腔医学研究》2018年第1期44-46,共3页Journal of Oral Science Research

摘　　要：目的:观测口腔鳞状上皮细胞癌(oral squamous cell carcinoma,OSCC)患者血清玻连蛋白(vitronectin,VTN)的表达情况,探讨其临床意义并分析其与口腔鳞状上皮癌分化程度及颈淋巴结转移之间的相关性。方法:应用蛋白印迹法检测2016年8月~2017年5月本院口腔颌面外科无淋巴结转移的口腔鳞癌患者26人,有淋巴结转移者18人,口腔黏膜中重度不典型增生患者8人,健康人群20人血清VTN表达情况,通过方差分析及LSD法检验各组血清中VTN的表达差异。结果:正常对照组VTN A值为(36849.10±1127.37),高于无淋巴结转移者(22518.12±1301.25),高于有淋巴结转移者(9459.28±629.58)(P<0.001),3组间差异均具有统计学意义;口腔黏膜中重度不典型增生患者VTN A值为(36725.50±1484.41)与对照组差异无统计学意义。另检测无淋巴结转移OSCC患者癌细胞分化程度不同的血清VTN表达情况:高分化鳞癌患者血清VTNA值为(59009.13±145.75),高于中分化者(39468.82±1135.61),高于低分化者(22437.00±1286.38)(P<0.001),3组间差异也均具有统计学意义。结论:血清玻连蛋白(VTN)与口腔鳞状上皮癌(OSCC)的发生、进展和淋巴结转移可能相关,测定血清VTN的表达水平可能对口腔鳞状上皮癌(OSCC)的诊断和疾病进展具有重要的参考价值。Objective：To observe the expression of blood serum vitronectin（VTN）in oral squamous cell carcinoma（OSCC）,and to explore its clinical significance and the degree of differentiation,as well as the correlation between cervical lymph node metastasis.Methods：Western blot method was used to detect VTN expression in 26 OSCC patients without lymph node metastasis,including 18 OSCC patients with lymph node metastasis,8 oral tissue atypical hyperplasia patients,20 healthy people from Department of Oral and Maxillofacial Surgery,affiliated Stomatological Hospital of Harbin Medical Universit from 2016,08,01-2017,05,30.Results：VTN was highly expressed in the blood serum of healthy people（36849.10±1127.37）than that of OSCC patients without lymph node metastasis（22518.12±1301.25）.VTN was also highly expressed in the blood serum of OSCC patients without lymph node than that of OSCC patients with lymph node metastasis（9459.28±629.58,P〈0.001）.VTN was36725.50±1484.41 in the blood serum of patients with atypical hyperplasia of oral mucosa.The expression of VTN in OSCC patients with different differentiated degree and without lymph node metastasis was analyzed.The results showed that VTN was highly expressed in the blood serum of high differentiated OSCC patients（59009.13±145.75）than that of moderately differentiated OSCC patients（39468.82±1135.61）.And its expression was also higher than that of poorly differentiated OSCC patients（22437.00±1286.38,P〈0.001）.Conclusion：VTN may be associated with the malignant transformation and mestastasis of OSCC,and expression of serum VTN may help OSCC diagnosis and disease progression assessment.

关 键 词：玻连蛋白 口腔鳞状上皮细胞癌 蛋白印迹法 

分 类 号：R739.8[医药卫生—肿瘤]