姜黄素联合PDTC体外抗白血病K562细胞的作用研究  

Study on Effect of Curcumin Combined with PDTC on Anti-leukemia K562 Cells in Vitro

作　　者：高荧[1] 于宜平[1] 苗久旺 张钦德[1] GAO Ying;YU Yi -ping;MIAO Jiu -wang;ZHANG De -qin(Shandong Junior College of Traditional Chinese Medicine, Yantai 264199, China)

机构地区：[1]山东中医药高等专科学校,山东烟台264199

出　　处：《云南中医中药杂志》2018年第1期72-74,共3页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：山东省中医药科技发展计划项目(NO:2011-151);山东省高等学校科技计划项目(NO:J11LF68);山东中医药高等专科学校科研项目(NO:2015ky009)

摘　　要：目的探讨姜黄素联合PDTC体外抗K562细胞的作用。方法 MTT法检测姜黄素单独或联用PDTC后对K562细胞增殖活性的影响;Hoechst33342染色法观察细胞凋亡形态;Western blot法检测Cleaved Caspase 3的表达活性。结果 10μmol·L^(-1)姜黄素联合25μmol·L^(-1)的PDTC后,可显著提高对K562细胞增殖的抑制活性,并显示出诱导细胞凋亡的形态学特征,使细胞Cleaved Caspase 3表达增加。结论姜黄素联合PDTC可增强抗K562细胞作用,机制与诱导K562细胞凋亡作用有关。Objective： To study the effect of curcumin combined with PDTC on anti - leukemia K562 cells in vitro. Methods： MTY assay was used to detect the effect of curcumin alone or in combination with PDTC on the proliferation of K562 cells. The morphology of apoptotic cells was observed by Hoechst33342 staining. The expression activity of Cleaved Caspase 3 was detected by Western blot. Results ： After treated with 10 μmol·L^-1 curcumin and 25μmol·L^-1 PDTC, the inhibitory activity on proliferation of K562 cells significantly in- creased, and the morphological characteristics of abduction apoptosis were also proved. The expression of Cleaved Caspase 3 increased. Conclusion： Curcumin combined with PDTC can enhance the anti- K562 cells and its mechanism is related to the abduction apoptosis of K562 cells.

关 键 词：姜黄素 吡咯烷二硫代氨基甲酸盐 细胞凋亡 CASPASE 3 

分 类 号：R285.5[医药卫生—中药学]