UPLC-MS/MS法测定猴头菌丝体中18种游离氨基酸的含量  

Determination of free amino acids in the mycelium of Hericium by UPLC-MS/MS

作　　者：唐翎[1] 魏伟[2] 赵勇 

机构地区：[1]中南大学湘雅医院药学部,长沙410008 [2]中南大学湘雅医院普外科,长沙410008 [3]湖南省药品检验研究院,长沙410001

出　　处：《药物分析杂志》2018年第1期112-117,共6页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立UPLC-MS/MS法测定猴头菌丝体中18种游离氨基酸的含量。方法:采用Hypersil GOLD C18色谱柱(100 mm×2.1 mm,1.9μm),以乙腈-0.1%甲酸溶液为流动相,梯度洗脱,流速0.2 mL·min^(-1);以电喷雾离子源(ESI)正离子模式将样品离子化,多反应监测(MRM)模式下对18种氨基酸进行测定。结果:18种氨基酸相关系数为0.999 3~1.000;平均回收率(n=6)为98.3%~100.0%,RSD在0.34%~1.5%。9批猴头菌丝体样品中,18种游离氨基酸总量在72.1~97.3 mg·g^(-1)之间。结论:本法可作为猴头菌丝体中游离氨基酸的含量测定方法。Objective：To establish a method for the determination of 18 free amino acids in the mycelium of Hericium by UPLC-MS/MS.Methods：The amino acids were separated on a Hypersil GOLD C18 column（100 mm×2.1 mm,1.9 μm） with acetonitrile-0.1% formic acid solution as mobile phase at a flow rate of 0.2 mL·min^-1 by gradient elution.Detection was carried out by positive eletrospray ionization（ESI） mass spectrometer in multiple reaction monitor（MRM） mode.Results：The correlation coefficients of 18 kinds of free amino acids ranged from 0.999 3 to 1.000.The average recoveries（n=6） were 98.3%^-100.0%,with RSD values of 0.34%^-1.5%.The total amounts of 18 free amino acids in 9 batches of samples were from 72.1 to 97.3 mg·g^-1.Conclusion：This method can be used to determine the content of 18 free amino acids in the mycelium of Hericium.

关 键 词：猴头菌丝体 游离氨基酸 含量测定 超高效液相色谱串联质谱法 多反应监测模式 

分 类 号：R917[医药卫生—药物分析学]