功能未知基因C6orf120缺失对自身免疫性肝炎大鼠CD4^+T细胞活化的影响  

Effects of deletion of unknown function gene C6orf120 on the activation of CD4^+ T cells in rats with autoimmune hepatitis

作　　者：张曼卡 马慧敏 张健[1] 叶小慧 何玲玲[4] 杨君茹 李鑫[1,2] 

机构地区：[1]北京大学地坛医院教学医院中西医结合中心,北京100015 [2]首都医科大学附属北京地坛医院中西医结合中心,北京100015 [3]北京大学地坛医院教学医院传染病研究所,北京100015 [4]首都医科大学附属北京地坛医院传染病研究所,北京100015

出　　处：《中国肝脏病杂志（电子版）》2017年第4期49-53,共5页Chinese Journal of Liver Diseases：Electronic Version

基　　金：十二五国家科技重大专项(2014ZX10005002-002);国家中医药行业科研专项(201507005);北京市中医药科技发展资金项目(JJ2015-72)

摘　　要：目的研究C6orf120基因缺失对自身免疫性肝炎大鼠CD4^+T细胞活化的影响。方法将野生型大鼠与C6orf120基因敲除大鼠分别随机分为5组,每组8只,以30 mg/kg刀豆蛋白A静脉注射诱导自身免疫性肝炎模型。于造模前后0小时、12小时、24小时、48小时和72小时处死大鼠,称量计算免疫器官指数,利用流式细胞术分析比较两种大鼠脾脏、外周血以及肝内淋巴细胞中CD4^+T细胞活化的差异,并比较外周血CD4^+/CD8^+T细胞比值的差异。结果 C6orf120基因敲除大鼠胸腺指数显著减小(F=20.868,P<0.001)。C6orf120基因敲除大鼠在刀豆蛋白A诱导12小时以及24小时后脾脏CD4^+T细胞活化增多(t值分别为3.538、2.547,P值分别为0.003、0.023);同时,外周血以及肝内淋巴细胞CD4^+T细胞活化增多(F值分别为33.801、55.015,P<0.001)。此外,C6orf120基因敲除后CD4^+/CD8^+显著上调(F=55.989,P<0.001)。结论本研究提示C6orf120基因缺失会促进自身免疫性肝炎大鼠CD4^+T细胞的增殖。Objective To investigate the effects of deletion of unknown function gene C6orf120 on the activation of CD4＋ T cells in autoimmune hepatitis rats. Methods Wild type and C6orf120 knockout rates were randomly divided into 5 groups, 8 rats in each group. Rates were given Con A 30 mg/kg by intravenous injection to establish autoimmune hepatitis model and sacrifced at 0 h, 12 h, 24 h, 48 h and 72 h before and after Con A challenging. The organs were weighed to calculate the index of immune organs. The CD4^＋ T cell activation in splenocytes, peripheral blood mononuclear cells and intrahepatic lymphocytes were analyzed by flow cytometry. Meanwhile, the ratio of CD4^＋/CD8^＋ cells in peripheral blood mononuclear cells was evaluated. Results The thymus index of C6orf120 knockout rats decreased signifcantly （F = 20.868 P〈0.001）. C6orf120 defciency increased CD4^＋ T cell activation in splenocytes in 12 h and 24 h （t = 3.538, 2.547; P = 0.003, 0.023）. Meanwhile, C6orf120 defciency increased CD4^＋ T cell activation in peripheral blood and intrahepatic lymphocytes （F = 33.801, 55.015; P 〈 0.001）. In addition, C6orf120 defciency signifcantly upregulated the CD4^＋/CD8^＋ ratio （F = 55.989, P 〈 0.001）. Conclusion C6orf120 defciency can upregulate the activation of CD4^＋ T cells in autoimmune hepatitis rats.

关 键 词：C6orf120 自身免疫性肝炎 CD4T细胞 刀豆蛋白A 

分 类 号：R575.1[医药卫生—消化系统]