PRRX1的表达对卵巢癌血管生成拟态形成及患者预后的影响  

Effect of PRRX1 expression on vasculogenic mimicry formation and prognosis in ovarian cancer

作　　者：范丹 宋文静[1,2] 张艳辉[3] 戚红[1] 古强[1,2] 董学易[1,2] 刘芳[1,2] 赵秀兰[1,2] 孙保存[1,2,3] 

机构地区：[1]天津医科大学病理学教研室,天津300070 [2]天津医科大学总医院病理科,天津300052 [3]天津医科大学肿瘤医院病理科,天津300060

出　　处：《天津医科大学学报》2018年第1期1-6,共6页Journal of Tianjin Medical University

基　　金：国家自然科学基金面上项目基金资助(81572872)

摘　　要：目的:检测配对相关同源框1(PRRX1)及其通路分子在卵巢癌中表达情况以及其与血管生成拟态(VM)的关系,推测PRRX1对卵巢癌患者预后的影响。方法:采用免疫组化法检测62例卵巢癌中PRRX1、Wnt5a、β-catenin的表达情况,采用CD31/PAS双染法检测卵巢癌中VM的情况。采用χ~2检验和Pearson相关分析来分析PRRX1与VM形成和临床病理资料之间的关系以及PRRX1与Wnt5a、β-catenin的关系。采用Kaplan-Meier法评估PRRX1对卵巢癌患者生存预后的影响。结果:PRRX1表达对卵巢癌患者的FIGO分期、转移及VM形成有影响且具有统计学意义(P<0.05),而对患者的年龄、肿瘤大小、组织学类型、肿瘤分化程度的影响无明显统计学差异(P>0.05)。PRRX1表达与β-catenin的核表达有关,与Wnt5a、β-catenin的浆表达无关。PRRX1阳性表达和有VM形成的患者生存时间比PRRX1阴性表达或无VM形成的患者短(P<0.05)且预后差;PRRX1阳性表达和β-catenin核阳性表达的患者生存时间短于PRRX1阴性表达或β-catenin核阴性表达的患者(P<0.05);而PRRX1阳性表达与Wnt5a阳性表达、β-catenin的浆阳性表达的患者生存时间与PRRX1阴性表达或Wnt5a阴性表达、β-catenin的浆阴性表达的患者生存时间相比,差别无意义(P>0.05)。结论:PRRX1通过Wnt/β-catenin信号通路调节VM形成从而影响卵巢癌患者预后,PRRX1可作为卵巢癌诊断和预后预测的指标。Objective： To investigate the paired related homeobox 1（PRRX1） and its pathway molecules expression in ovarian cancer and its relationship with the vasculogenic mimicry（VM） formation, so as to explore PRRX1 effect on metastasis and prognosis in ovarian cancer.Methods： The expressions of PRRX1,Wnt5 a, β-catenin as well as VM in ovarian cancer samples from 62 cases were examined by immunohistochemical and CD31/periodic acid-Schiff double staining. And the relationships between PRRX1 and VM formation, Wnt5 a,β-catenin were assessed by χ^2 and pearson tests. The prognostic values of PRRX1 were measured by Kaplan-Meier survival analysis.Results： PRRX1 expression was associated with FIGO stage, metastasis and VM formation（P〈0.05）. No significant correlations were found between PRRX1 and age, tumor size, histological type, degree of differentiation of the tumor（P〈0.05）. PRRX1 overexpression was found to have the relationship with β-catenin nuclear expression but showed no relationship with Wnt5 a and β-catenin cytoplasm expression. Both PRRX1 expression and VM formation were correlated with ovarian cancer metastasis and prognosis. Kaplan-Meier survival analysis showed that patients with PRRX1 expression and VM formation had shorter survival than PRRX1-or VM-patients, respectively. And poor prognosis was observed. PRRX1 expression and β-catenin nuclear expression had shorter survival than PRRX1-or β-catenin nuclear negative expression（P〈0.05）, where this relationship showed no significance between PRRX1 and Wnt5 a, β-catenin cytoplasm expression（P〈0.05）. Conclusion： PRRX1 could regulate VM formation in ovarian cancer through the activation of Wnt/β-catenin signaling,indicating poor prognosis, so PRRX1 might be a candidate marker for the diagnosis and prognosis predictor of ovarian cancer.

关 键 词：卵巢癌 配对相关同源框1 血管生成拟态 Β-CATENIN WNT5A 

分 类 号：R737.31[医药卫生—肿瘤]