小鼠小细胞肺癌模型的建立  

Establishment of a mouse model of pulmonary small cell lung cancer

作　　者：王子乔 张雪茜 孟凡荣 刘喆 

机构地区：[1]天津医科大学医学检验学院,天津300070

出　　处：《天津医科大学学报》2018年第1期29-31,共3页Journal of Tianjin Medical University

摘　　要：目的:建立小鼠小细胞肺癌动物模型,为进一步研究小细胞肺癌的发展进程以及化疗药物的筛选提供实验平台。方法:采用Trp53^(loxP/loxP);Rb1^(loxP/loxP)小鼠肺部插管吸入Adeno-Cre(Ad-Cre)重组腺病毒的方法建立小鼠小细胞肺癌模型,通过对模型小鼠肺组织进行石蜡切片以及HE染色,并通过免疫组化等方法检测肺组织中神经内分泌特异性标记蛋白CgA、Cgrp的表达情况。结果:成功建立了小鼠小细胞肺癌的模型,模型小鼠肺组织中TTF-1、CgA、Cgrp的表达水平显著升高。结论:成功建立小鼠小细胞肺癌动物模型,为后续研究人的小细胞肺癌发病机制以及相关化疗药物的筛选提供了实验手段。Objective：To establish a model of small cell lung cancer in mice to study the progression of small cell lung cancer and to provide an experiment platform for the screening of chemotherapy drugs.Methods：The small cell lung cancer model was established by intratracheal injection of Adeno-Cre（Ad-Cre）into Trp53^loxP/loxP;Rb1^loxP/loxPmice.The lung tissues of model mice were sectioned and stained with HE.Immunochemistry of neuroendocrine cell-specific markers CgA and Cgrp was performed on lung sections.Results：The small cell lung cancer model was successfully established and a significant increase was observed in the expression of TTF-1,CgA and Cgrp.Conclusion：This mice model provides an experiment method for the study of pathogenesis of human small cell lung cancer and the screening of chemotherapy drugs.

关 键 词：Ad-Cre Trp53^loxP/loxP Rb1^loxP/loxP小鼠 小细胞肺癌 动物模型 

分 类 号：R734.2[医药卫生—肿瘤]