应用植入遥测技术对恒河猴部分生理指标的观测  

Observation of several physiological indexes of rhesus monkeys by implantable telemetry technology

作　　者：卢祺炯[1] 陈敏华[1] 徐聪 邓军[1] 徐志伟[1] 宣尧仙[1] 

机构地区：[1]长三角绿色制药协同创新中心药物安全性评价团队,浙江省医学科学院安全性评价研究中心,杭州310053

出　　处：《中国比较医学杂志》2018年第1期100-107,共8页Chinese Journal of Comparative Medicine

摘　　要：目的应用植入遥测技术观测清醒恒河猴呼吸、血压、心电、体温等生理指标的昼夜变化规律和应激时的变化。方法取3~5岁雌雄各半的8只恒河猴行遥测植入子植入手术,恢复3周后用DSI遥测系统获取清醒无束缚恒河猴连续24 h的呼吸、血压、心电、体温数据,用Ponemah软件分析上述指标并统计实验结果。结果恒河猴的部分心电指标存在明显的昼夜差异(P<0.05或P<0.01),其中心率(HR)的昼夜均值在每分钟155.0~122.4次之间波动;呼吸频率间期(RR-I)为410.8~535.7 ms;T波幅度(T-A)为0.181~0.157 m V;PR间期(PRI)为80.4~87.4;QT间期(QT-I)为224.8~263.9 ms;校正QT间期(QTcb)为352.3~366.7 ms。猴的血压与呼吸指标白昼均值显著高于夜晚(P<0.01),其中收缩压(SYS)昼夜波动范围为144.6~131.6 mm Hg;舒张压(DIA)为99.8~89.9 mm Hg;平均动脉压(MAP)为121.5~110.2 mm Hg;潮气量(TV)为64.5~36.6 m L;分钟通气量(MV)为1931.9~920.1 m L/min;呼吸频率(RR)为每分钟32.3~25.4次。实验室工作人员清扫、喂食活动对猴有一定的应激影响。结论应用植入遥测技术观察到的恒河猴呼吸、血压、心电、体温等生理指标基本均有明显的昼夜节律变化,呼吸、血压、心电、体温等指标白昼均高于夜晚,符合正常恒河猴的生理周期特性。经过驯养的恒河猴在饥饿状态下受工作人员的喂食、清扫活动的应激影响比较强烈。应用植入遥测技术可以对清醒无束缚状态下恒河猴心电、血压、呼吸、体温等进行连续监测,能真实地反映恒河猴24 h内上述生理指标的变化规律,为恒河猴在药理毒理学研究中的应用提供参考。同时应用植入遥测技术,有助于提高药物安全药理学研究的效率,减少动物的使用数量,符合3R原则。Objective To observe the changes of circadian characteristics and stress-response-related physiological parameters including respiration,blood pressure,electrocardiography and body temperature of conscious rhesus monkeys by implantable telemetry technique. Methods Surgery was performed on 8 rhesus monkeys（ half male and half female,3-5 years old） for implantation of a telemetry transmitter. After 3 weeks of recovery,the physiological parameters of respiration,blood pressure,electrocardiography and body temperature of the conscious rhesus monkeys without binding were automatically recorded by a DSI telemetry system and the data were analyzed by the Ponemahsoftware. Results Some electrocardiographic indexes showed significant differences at daytime and nighttime（ P〈0. 05 or P〈0. 01） including mean heart rate（ HR）（ 155. 0-122. 4 times/min）,respiratory rate interval（ RR-I）（ 410. 8-535. 7 ms）,T-wave amplitude（ T-A）（ 0. 181-0. 157 m V）,PR interval（ PR-I）（ 80. 4-87. 4 ms）,QT interval（ QTI）（ 224. 8-263. 9 ms）,and corrected QTcb interval（ QTcb）（ 352. 3-366. 7 ms）. The indexes of blood pressure and respiration at daytime were significantly higher than those at nighttime（ P〈0. 01）,including the mean systolic pressure（ SYS） at daytime and nighttime（ 144. 6-131. 6 mm Hg）,diastolic pressure（ DIA）（ 99. 8-89. 9 mm Hg）,mean arterial pressure（ MAP）（ 121. 5-110. 2 mm Hg）,tidal volume（ TV）（ 64. 5-36. 6 m L）,minute ventilation（ MV）（ 1931. 9-920. 1 m L/min）,and respiratory rate（ RR）（ 32. 3-25. 4 times/min）. Cleaning and feeding activities of the laboratory staff at 9： 00 a. m. and 2： 00 p. m. had a certain effect on the stress-responses in the monkeys. Conclusions The parameters of respiration,blood pressure,electrocardiography and body temperature of the conscious rhesus macaques observed by implanted telemetry system show obvious circadian changes,which can truly reflect the changes of physiological indexes at daytim

关 键 词：安全药理学 植入遥测技术 恒河猴 生理指标 

分 类 号：R-33[医药卫生]