针刺治疗慢性心肌缺血小型猪模型系列方法的可行性探讨  

Feasibility study of acupuncture treatment on the miniature pig model of chronic myocardial ischemia

作　　者：张金铃[1] 田毅[2] 荣培晶[1] 方继良[3] 王瑜 石力[1] 贾术永[1] 何勋[1] 雷洪涛[4] 孟宏 

机构地区：[1]中国中医科学院针灸研究所,北京100700 [2]北京协和医学院,中国医学科学院阜外医院,北京100037 [3]中国中医科学院广安门医院,北京100053 [4]中国中医科学院医学实验中心,北京100700 [5]北京工商大学理学院化妆品系,北京100048

出　　处：《中国比较医学杂志》2018年第1期108-112,共5页Chinese Journal of Comparative Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(编号：2012CB518503); 国自然青年基金(编号：81102650); 北京工商大学北京市植物资源研究开发重点实验室开放课题基金资助课题

摘　　要：目的探讨埋针治疗手术放置Ameroid缩窄环于左前降支的小型猪慢性心肌缺血模型系列方法的可行性。方法在巴马小型猪左冠状动脉左前降支放置Ameroid缩窄环制备小型猪慢性心肌缺血模型,随机分为治疗组(针刺"内关"穴)和对照组(针刺"足三里"穴),分别进行"内关"和"足三里"穴埋针并电针治疗,6周后取材。结果术后大体病理显示两组均出现缺血区域,针刺"足三里"穴缺血面积大于"内关"穴,心肌细胞超微结构显示两组均有线粒体空泡化、纤维化现象。结论针刺心包经"内关"穴可以通过减轻心肌缺血起到保护心肌的效应,但不能抑制已经存在的心肌缺血损伤。说明手术放置Ameroid缩窄环于冠脉左前降支建立大型实验动物猪慢性心肌缺血模型,并采用埋针进行治疗的方法是可行的。Objective To explore the feasibility of needle-embedding therapy in the treatment of chronic myocardial ischemia using a miniature pig model established by placement of an Ameroid constrictor at the left anterior descending branch（ LAD） of coronary artery in Bama miniature pigs during surgery. Methods The miniature pig model of chronic myocardial ischemia was established by placement of an Ameroid constrictor at the left anterior descending branch of the left coronary artery in Bama miniature pigs. The pig models were randomly divided into the treatment group（ the＂Neiguan＂ group） and the control group（ the ＂Zusanli ＂ group）, and were treated with needle-embeddingelectroacupuncture at the ＂Neiguan＂（ PC6） and ＂Zusanli＂（ ST36） acupoints,respectively. Myocardial samples were taken at 6 weeks after surgery for light and electron microscopic examinations. Results Gross pathology showed that ischemic area in the myocardium appeared in both experimental groups. The ischemic area in the ＂Zusanli＂group was larger than that of the＂Neiguan＂group. Histopathology showed that the acupuncture treatment at the＂Neiguan＂acupoint reduced the ischemic injury in the pig myocardial tissues. Ultrastructural observation of the myocardium showed mitochondrial vacuolization in cardiomyocytes and myocardial fibrosis in both groups. Conclusions Acupuncture therapy at the ＂Neiguan＂acupoint of pericardial channel may exert protective effect on the myocardial ischemia by reducing the ischemia-injury of cardiomyocytes,but can not inhibit the already existed ischemia-induced cardiomyocytic injuries. Our findings suggest that the establishment of miniature pig model of chronic myocardial ischemia by surgically placing an Ameroid constrictor on the left anterior descending branch of left coronary artery and the needle-embedding in acupoints is feasible for the treatment of chronic myocardial ischemia in this pig model.

关 键 词：大型实验动物 巴马小型猪 慢性心肌缺血 埋针并电针 大体病理 电镜超微结构 

分 类 号：R-33[医药卫生]