信号转导与转录激活蛋白活化抑制蛋白1的表达与胃癌微环境转移结构关系及预后  

Correlation of expression of protein inhibitor of activated signal transducer and activator of transcription 1 with tumor microenvironment of metastasisin gastric cancer and its significance in clinical prognosis

作　　者：陈平 俞骁珺 谷雷雷 张梦茵 朱时燕 吴云林 CHEN Ping;YU Xiaojun;GU Leilei;ZHANG Mengyin;ZHU Shiyan;WU Yunlin(Department of Gastroenterology, Ruijin Hospital North Branch, Shanghai Jiao Tong University School of Medicine, Shanghai 230001, China)

机构地区：[1]上海交通大学医学院附属瑞金医院北院消化内科,上海201801

出　　处：《内科理论与实践》2017年第6期365-370,共6页Journal of Internal Medicine Concepts & Practice

基　　金：上海市卫生和计划生育委员会科研课题(项目编号:201640231)

摘　　要：目的:通过检测信号转导与转录激活蛋白(STAT)活化抑制蛋白1(PIAS1)在胃癌中的表达情况,分析其与胃癌微环境的关系及判断其对疾病预后的价值。方法:收集浅表性胃炎、萎缩性胃炎、胃癌前病变标本各20份,胃腺癌(包括癌旁组织、腺癌)标本75份,通过免疫组织化学(免疫组化)测定PIAS1、CD34、CD68,了解各组间巨噬细胞、肿瘤微环境转移(TMEM)结构的数目,分析各组患者PIAS1、巨噬细胞及TMEM的表达差异,并分析临床参数(性别、年龄、分期、大小和分化)与上述指标之间关系。结果:在浅表性胃炎、萎缩性胃炎、癌前病变、癌旁及胃癌组织中,PIAS1的阳性表达率分别为90.0%、80.0%、80.0%、48.0%、26.7%,组间比较差异有统计学意义(P<0.05),免疫组化测定显示巨噬细胞数目分别为(7.45±2.28)、(8.35±2.70)、(8.95±2.56)、(8.80±2.13)、(12.64±3.84)个/高倍视野(HP)。其中远处转移和无远处转移胃癌患者巨噬细胞数分别为(13.96±3.81)、(11.80±3.66)个/HP,TMEM为4.17±1.55和3.26±1.32,差异均有统计学意义(均P<0.05)。肿瘤的不同分期间PIAS1、巨噬细胞和TMEM比较,差异有统计学意义(均P<0.05)。PIAS1阳性和阴性患者中,巨噬细胞计数和TMEM表达率差异有统计学意义(P<0.05)。结论 :PIAS1在胃癌组织中低表达,并与TMEM结构密切相关,其可能成为疾病预后的客观指标及治疗靶点之一。Objective To investigate the correlation of expression of protein inhibitor of activated signal transducer and activator of transcription 1（ PIAS1） with tumor microenvironment of metastasis（ TMEM） of gastric cancer and its significance in clinical prognosis. Methods The expression of PIAS1, number of macrophagesand TMEM in 20 specimens each of superficial gastritis, atrophic gastritis, precancerous lesion, and 75 specimens of gastric cancer and adjacent normal tissue were detected immunohistochemically. TMEM was evaluated using anti-CD34 antibody as endothelial marker and anti-CD68 antibody as macrophage marker. The correlations between PIAS1 expression and number of macrophages,TMEM, clinical data including age, sex, stage, size and differentiation of tumor were analyzed. Results The positive expression rate of PIAS1 was lower and number of macrophages was higher in gastric cancer tissue than those in superficial gastritis, atrophic gastritis, precancerous lesion, adjacent normal tissues（26.7% vs 90.0%, 80.0%, 80.0%,48.0%, respectively）, [（12.64 ±3.84） vs（7.45 ±2.28）,（8.35 ±2.70）,（8.95 ±2.56）,（8.80 ±2.13）/high power field（HP）,respectively], the differences were statistically significant（P0.05）. The number of macrophages and TMEM were higher in gastric cancer with metastasis than those without metastasis [（13.96±3.81） vs（11.80±3.66）/HP,（4.17±1.55） vs（3.26±1.32）].PIAS1 expression, number of macrophages and TMEM showed significant differences between different stages of gastric tissue（P 0.05）. Number of macrophages and TMEM showed significant differences between those with positive and negative of PIAS1 expression（all P 0.05）. Conclusions Lower-expression of PIAS1 was correlated with TMEM and prognosis of gastric cancer, which might be a prognosis marker and the therapeutic target of gastric cancer.

关 键 词：胃癌 信号转导与转录激活蛋白活化抑制蛋白1 肿瘤微环境转移 预后 

分 类 号：R735.2[医药卫生—肿瘤]