IL-2激活的NK细胞对胶质瘤的杀伤作用  

The killing effect of IL-2 activated NK cells onglioma

作　　者：吴帅帅 乔小放 赵红梅[2] 王晓科[1] 王海亮[1] 于翔[1] 刘军[1] 

机构地区：[1]吉林大学第二医院,吉林长春130041 [2]长春市第二医院,吉林长春130062

出　　处：《中风与神经疾病杂志》2018年第1期18-21,共4页Journal of Apoplexy and Nervous Diseases

基　　金：吉林省科技发展计划项目(No.20150101193JC)

摘　　要：目的研究白细胞介素2(IL-2)激活的自然杀伤细胞(NK细胞)对动物模型中胶质瘤的杀伤作用。方法通过将裸鼠分为7组A:无菌裸鼠对照组;B:体内移植GBM组织组;C:体内注射IL-2组;D:体内注射NK细胞组;E:同时移植GBM组织和NK细胞组;F:同时移植IL-2和NK细胞组;G:GBM组织、NK细胞和IL-2移植组。观察各组裸鼠生长情况、裸鼠肿瘤生长情况、肿瘤出现时间、肿瘤生长情况(体积、重量)等数据,并进行统计学分析。结果 NK细胞对GBM具有抑制、杀伤作用,同时IL-2能促进NK细胞对GBM的抑制及杀伤作用的能力,可以延缓胶质瘤的生长。结论 NK细胞对人脑胶质瘤具有抑制杀伤作用,并为临床免疫疗法治疗胶质瘤的研究奠定基础。Objective To investigate the effects of interleukin-2（ IL-2） activated NK cells on gliomas in animal models. Methods The nude mice were divided into seven groups. A： sterile nude mice control group; B： in vivo transplantation GBM group; C： in vivo injection of IL-2 group; D： injection of NK cells group; E： simultaneous transplantation of BGM tissue and NK cells group; F： simultaneous transplantation of IL-2 and NK cells group; G： simultaneoustransplantation of BGM,NK cells and IL-2 group. To observe the growth of nude mice,the growth of tumor in nude mice,the emergence time of the tumor,the growth of the tumor（ size and weight） data etc,and carry out statistical analysis. Results NK cells have hadinhibition and killing effect on GBM,and IL-2 can promote the inhibition and killing effect of NK cells on GBM,and can coulddelay the growth of glioma. Conclusion NK cells have the effect of inhibiting the human brain glioma and lay the foundation for the clinical immunotherapy of glioma.

关 键 词：脑胶质瘤 NK细胞 IL-2 

分 类 号：R739.41[医药卫生—肿瘤]