利卡汀联合吡柔比星TACE对不可切除肝癌组织中细胞凋亡及上皮间质转化的影响  

Effect of licartin combined with pirarubicin TACE on apoptosis and epithelial-mesenchymal transition in unresectable liver cancer tissue

作　　者：张嘉诚 田艳云 杨超 马军朋 蒋富强 杨剑 卢伟 

机构地区：[1]北京海军总医院介入医学科,北京100048

出　　处：《海南医学院学报》2018年第2期246-249,共4页Journal of Hainan Medical University

基　　金：国家自然科学基金(30670612);中华国际医学交流基金会(Z-2014-06-15322);海军后勤部(HJHQ-20130987)~~

摘　　要：目的:研究利卡汀联合吡柔比星肝动脉化疗栓塞术(TACE)对不可切除肝癌组织中细胞凋亡及上皮间质转化的影响。方法:选择在本院就诊的不可切除肝癌患者,并随机分为两组,利卡汀组接受利卡汀联合TACE治疗,对照组接受单纯TACE治疗。治疗前后测定肝癌病灶中抑癌基因、自噬标志基因及上皮间质转化标志基因的表达量。结果:手术后,两组患者肝癌病灶中RNF180、EBLN2、ALKBH5、Merlin、Beclin-1、LC3-II、TRAF-6、E-cadherin的蛋白表达量均显著高于手术前,Shh、Gli1、Snail、Twist、N-cadherin、Vimentin的蛋白表达量显著低于手术前且利卡汀组患者肝癌病灶中RNF180、EBLN2、ALKBH5、Merlin、Beclin-1、LC3-II、TRAF-6、E-cadherin的蛋白表达量显著高于对照组,Shh、Gli1、Snail、Twist、N-cadherin、Vimentin的蛋白表达量显著低于对照组,差异均有统计学意义(P<0.05)。结论:利卡汀联合吡柔比星TACE治疗不可切除肝癌组织能够更为有效地诱导细胞凋亡及自噬、抑制细胞上皮间质转化。Objective:To study the effect of licartin combined with pirarubicin transcatheter arterial chemoembolization(TACE)on apoptosis and epithelial-mesenchymal transition in unresectable liver cancer tissue.Methods:Patients with unresectable liver cancer who were treated in Navy General Hospital between May 2014 and October 2016 were selected and randomly divided into two groups,the licartin group received licartin combined with TACE therapy,and control group received TACE therapy alone.The expression of tumor suppressor genes,autophagy marker genes and epithelial-mesenchymal transition marker genes in liver cancer lesions were determined before and after treatment.Results:After operation,RNF180,EBLN2,ALKBH5,Merlin,Beclin-1,LC3-Ⅱ,TRAF-6 and E-cadherin protein expression in liver cancer lesions of both groups of patients were significantly higher than those before operation while Shh,Gli1,Snail,Twist,N-cadherin and Vimentin protein expression were significantly lower than those before operation,and RNF180,EBLN2,ALKBH5,Merlin,Beclin-1,LC3-II,TRAF-6 and E-cadherin protein expression in liver cancer lesions of licartin group were significantly higher than those of control group while Shh,Gli1,Snail,Twist,N-cadherin and Vimentin protein expression were significantly lower than those of control group.Conclusion:Licartin combined with pirarubicin TACE for unresectable liver cancer can more effectively induce apoptosis and autophagy,and inhibit epithelial-mesenchymal transition.

关 键 词：肝癌 利卡汀 肝动脉化疗栓塞术 凋亡 上皮间质转化 

分 类 号：R735.7[医药卫生—肿瘤]