肺炎克雷伯菌TH6菌株的生物学特性及生物膜形成分析  

Biological characteristics and biofilm formation of Klebsiella pneumoniae TH6 strain

作　　者：李博星 陆静 程伟伟 李志西[1] 袁静[2] LI Bo-xing;LU Jing;CHENG Wei-wei;LI Zhi-xi;YUAN Jing(College of North West Agriculture and Forestry University,Institute of Disease Control and Prevention,Academy of Military Medical Science)

机构地区：[1]西北农林科技大学,陕西杨陵712100 [2]军事医学科学院疾病预防控制所传染病控制中心,北京100071

出　　处：《军事医学》2017年第10期805-809,共5页Military Medical Sciences

基　　金：国家自然科学基金资助项目(31200137)

摘　　要：目的从临床炎症性肠炎患者粪便中分离1株肺炎克雷伯菌株(Klebsiella pneumoniae),探究其生物学特性及生物膜形成的特点。方法对TH6菌株进行16S RNA鉴定、MALDI-TOF的质谱鉴定以及全自动生化检测,确定为肺炎克雷伯杆菌。对其形态,生长速率,生物膜形成,荚膜毒力因子等生物学特性进行分析。结果与结论在透射电镜下TH6菌株为短杆状,具有荚膜,荚膜血清型实验结果显示为K57型。K.pneumonia TH6在有氧条件下生长速度非常快,为标准菌株ATCC2146和ATCC1705的1.6倍,在1 h时即可达到对数生长期。在激光共聚焦显微镜下分别观察4、8、12、24、48、72、96和120 h内的生物膜形成状况,发现该菌株在培养72 h后形成生物膜。K.pneumonia TH6的生长速度快、生物膜的形成等特点均表明该菌适应新环境能力强,一旦在体内定植则难以根治。Objective To explore the biological characteristics and biofilm formation of a strain of Klebsiella pneumoniae isolated from the stool of a patient with clinical inflammatory bowel disease. Methods The TH6 strain was identified as Klebsiella pneumoniae by 16 S RNA,MALDI-TOF and automatic biochemical instrument. The morphology,growth rate,biofilm formation,capsid virulence factors and other biological characteristics were analyzed. Results and Conclusion The TH6 strain was short rod-shaped with a capsule under the transmission electron microscope,and was of K57 type according to the capsule serotype test. K. pneumonia TH6 grew rapidly under aerobic and anaerobic conditions,almost at twice the rate of standard strain ATCC2146,and reached the logarithmic growth phase at 2 h. The strain had multi-drug resistance. Biofilm formation was observed under a confocal laser scanning microscope（ CLSM） at 4,8,12,24,48,72,96 and 120 h and the biofilm was formed at 72 h. The ability of fast growth,biofilm formation and other characteristics of K. pneumonia TH6 showed that this strain has a strong ability to adapt to the new environment is difficult to cure after colonization in vivo.

关 键 词：肺炎克雷伯菌 生长曲线 荚膜分型 生物膜 

分 类 号：R446.5[医药卫生—诊断学]