黄皮种子中酰胺类生物碱及其杀线虫活性研究  

Amide Alkaloids from the Seeds of Clausena lansium and Their Nematicidal Activities

作　　者：范玉娇 陈惠琴[2] 梅文莉[2] 许书慧 周丽曼[2] 黄绵佳 戴好富[2] 

机构地区：[1]海南大学热带农林学院,海口570228 [2]中国热带农业科学院热带生物技术研究所,农业部热带作物生物学与遗传资源利用重点实验室,海口571101 [3]海口市人民医院药学部,海口570208

出　　处：《热带亚热带植物学报》2018年第1期85-91,共7页Journal of Tropical and Subtropical Botany

基　　金：海南省卫生计生科教项目(16A200125); 海南省重点研发计划项目(ZDYF2016140); 中国热带农业科学院基本科研业务费专项资金(17CXTD-15)资助

摘　　要：为了解黄皮种子中的酰胺类生物碱及其杀线虫活性,运用多种色谱学及波谱学方法分离并鉴定了10个酰胺类生物碱,分别为:N-甲基桂皮酰胺(1),clausenalansamide A(2),3-dehydroxy-3-methoxyl-clausenalansamide A(3),clausenalansamide B(4),黄皮新肉桂酰胺B(5),N-(2-苯乙基)肉桂酰胺(6),2′-dehydroxy-2′-oxo-clausenalansamide B(7),lansamide-7(8),homoclausenamide(9),1,5-dihydro-5-hydroxy-1-methyl-3,5-diphenyl-2H-pyrrol-2-one(10)。其中,化合物3,7,10为新天然产物。首次对黄皮种子中的酰胺类生物碱2~8进行全齿复活线虫致死活性的测试,发现所测化合物均有致死活性,其中,化合物2,3,5和8有较强的致死活性,且均优于阳性对照除线磷,可为相关农药的研发提供科学依据。In order to understand the nematicidal activities of amide alkaloids from the seeds of Clausena lansium, ten amide alkaloids were isolated by comprehensive chromatographic techniques. On the basis of spectral data, their structures were identified as： N-methyl-cinnamamide（1）, clausenalansamide A（2）, 3-dehydroxy-3-methoxyl-clausenalansamide A（3）, clausenalansamide B（4）, lansiumamide B（5）, N-2-phenylethyl-cinnamamide（6）, 2′-dehydroxy-2′-oxo-clausenalansamide B（7）, lansamide-7（8）, homoclausenamide（9）, 1,5-dihydro-5-hydroxy-1-methyl-3,5-diphenyl-2 H-pyrrol-2-one（10）. Among them, compounds 3, 7 and 10 were new natural products. Meanwhile, the nematicidal activity against Panagrellus redivivusl was tested for the first time. All of them showed the nematicidal activity, and compounds 2, 3, 5 and 8 presented stronger activities than dichlorofenthion, providing scientific evidence for the development of new pesticide.

关 键 词：黄皮 种子 酰胺类生物碱 全齿复活线虫 致死活性 

分 类 号：S482[农业科学—农药学]