mPGES-1、NF-κB p65在弥漫大B细胞淋巴瘤中的表达及临床意义  

Expression and Clinical Significance of mPGES-1 and NF-κB p65 in Diffuse Large B Cell Lymphoma

作　　者：何志媛 谢双锋[1] 聂大年[1] 尹松梅[1] 何璋海 肖洁[1] 马丽萍[1] 李益清[1] 

机构地区：[1]中山大学孙逸仙纪念医院血液内科,广东广州510120 [2]中山大学孙逸仙纪念医院病理科,广东广州510120

出　　处：《中山大学学报（医学版）》2018年第1期73-81,共9页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然青年科学基金(81200342);广东省公益研究与能力建设专项资金(2014A020212085);广东省自然科学基金基础与应用基础研究专项自由申请项目(2016A030313360)

摘　　要：【目的】检测膜结合型前列腺素E2合酶1(mPGES-1)和核转录因子κB p65(NF-κB p65)在弥漫大B细胞淋巴瘤(DLBCL)组织中的表达,探究两者与主要临床特征及预后关系,分析它们的潜在的临床价值。【方法】采用免疫组织化学方法,检测83例DLBCL组织中mPGES-1和NF-κB p65的表达,分析两者与临床特征及预后关系。【结果】DLBCL组织中mPGES-1、NF-κB p65高表达所占的比例分别为65.1%(54/83)和73.5%(61/83),且两者的表达水平呈正相关(P<0.05)。这两个蛋白的高表达与B淋巴细胞瘤/白血病蛋白-2(BCL-2)、高Ki-67、高乳酸脱氢酶(LDH)、淋巴结外侵犯、临床晚期和国际预后指数(IPI)评分高有关(P<0.05)。此外,NF-κB p65与多发性骨髓瘤癌基因1(MUM1)、病理类型也有关(P<0.05)。生存分析提示,mPGES-1和NF-κB p65是总生存期(OS)的不良因素,其中NF-κB p65是OS的独立预后因素(P<0.05)。【结论】mPGES-1和NF-κB p65在DLBCL高表达,且两者之间呈正相关,这两种蛋白与DLBCL患者的肿瘤进展和不良预后密切相关。【Objective】Examine the expression of microsomal prostaglandin E synthase-1(mPGES-1)and nuclear transcription factor kappa B p65(NF-κB p65)in diffuse large B cell lymphoma(DLBCL),assessing their correlation with clinical variables,prognosis and potential clinical valve.【Methods】The immunohistochemistry was uesd to investigate the expression of mPGES-1 and NF-κB p65 in 83 DLBCL patiens' tissues. The relationship between these two proteins and the clinical variables and prognosis of these patients was evaluated.【Results】The high expression of mPGES-1 and NF-κB p65 were observed in 65.1%(54/83)and 73.5%(61/83)cases of DLBCL,respectively. The expression level of NF-κB p65 was positively correlated with mPGES-1 expression(P<0.05). The expression of these two proteins was found to be significantly associated with B cell lymphoma/leukemia-2 protein(BCL-2),higher expression of Ki67,higher lactate dehydrogenase(LDH),more extranodal lesions,advanced Ann Arbor stage and higher international prognostic index(IPI)score(P<0.05). In addition,NF-κB p65 was related with multiple myeloma oncogene 1(MUM1),pathological type(P<0.05).Both mPGES-1 and NF-κB p65 overexpression was correlated with worse overall survival(OS)while NF-κB p65 was an independent prognostic factor for OS of DLBCL(P<0.05).【Conclusions】mPGES-1 and NF-κB p65 were highly expressed in DLBCL and closely linked with each other. The overexpression of mPGES-1 and NF-κB p65 was correlated with tumor progression and unfavorable prognosis in patients with DLBCL.

关 键 词：膜结合型前列腺素E2合酶1 核转录因子κBp65 弥漫大B细胞淋巴瘤 临床意义 预后 

分 类 号：R733.4[医药卫生—肿瘤]