香紫苏油主成分含量测定及其香气贡献评价  

Main components determination and their contribution to odor in clary sage( Salvia sclarea L. ) oil

作　　者：白冰[1] 楚首道 杨靖[1] 张改红[1] 贾春晓[2] 毛多斌[1] 

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450001 [2]郑州轻工业学院材料与化学工程学院,河南郑州450001

出　　处：《轻工学报》2018年第1期7-12,共6页Journal of Light Industry

基　　金：中国烟草总公司重点科技项目(110201402033)

摘　　要：以苯甲醇为内标物,选用HP-5MS毛细管色谱柱,采用GC-MS法对香紫苏油中10种主要成分进行含量测定,并对其香气阈值和香气活力值进行测定和计算.结果表明:该方法可使样品中各组分很好地分离,线性方程相关系数大于0.999,加标回收率为90.6%~97.2%,检出限为9.5~232.8 ng/L,说明该方法重复性和灵敏度良好;香紫苏油中芳樟醇和乙酸芳樟酯的含量分别为20.3%和42.7%,香气阈值较低且香气活力值远高于其他成分,对香紫苏油香气贡献最大,与嗅香评价结果一致.The selected ion monitoring（ SIM） was used as the mass spectrum signal acquisition mode. UsingHP-5 MS capillary column,a method for determination of ten compounds in clary sage（ Salvia sclarea L.） oil has been developed. Odor threshold values of ten compounds were determined,and odor active values were obtained. The results showed that： Benzyl alcohol was used as an internal standard. The samples were separated on a DB-5 capillary column. The selected ion monitoring（ SIM） was used as the mass spectrum signal acquisition mode. The components of the sample were well separated in the experimental conditions above,and had good linearity with the correlation coefficients higher than 0. 999. The average recoveries were in the range of90. 6% ~ 97. 2%. The limits of detection（ S/N≥10） were in the range of 9. 5 ~ 232. 8 ng/L,which illustrated the good repetability and sensibility of the method. The compounds in clary sage oil with high odor active value were linaloolwith the content of 20. 3% and linalyl acetate with the content of 42. 7%.

关 键 词：香紫苏油 气相色谱-质谱联用 香气阈值 香气活力值 

分 类 号：TS47[农业科学—烟草工业]