多发性骨髓瘤患者外周血单个核细胞中白细胞介素-17的表达及意义  

Expression of interleukin-17 in peripheral blood mononuclear cells of patients with multiple myeloma

作　　者：秦卫玲[1] 邢会茹 赵玉洁 武金日 窦敬芳[1] 贺立山[2] 

机构地区：[1]新乡医学院第三附属医院血液内科,河南新乡453003 [2]新乡医学院第一附属医院血液内科,河南卫辉453100

出　　处：《新乡医学院学报》2018年第2期140-142,共3页Journal of Xinxiang Medical University

摘　　要：目的探讨多发性骨髓瘤(MM)患者外周血单个核细胞(PBMC)中白细胞介素-17(IL-17)的表达及意义。方法选取新乡医学院第三附属医院血液科2016年1月至2017年1月确诊的30例MM患者为观察组,选择与观察组患者性别、年龄相匹配的健康献血者30例作为对照组。应用密度-梯度离心法分别获取观察组及对照组受试者的PBMC,采用酶联免疫吸附试验检测2组受试者PBMC培养液上清液中IL-17水平;采用实时定量聚合酶链反应检测2组受试者PBMC中IL-17mRNA表达;将对照组受试者的PBMC与观察组患者的血清进行共培养,然后使用扫描电镜观察PBMC的形态。结果观察组和对照组患者PBMC培养液上清液中IL-17水平分别为(30.79±4.96)、(10.10±4.15)ng·L^(-1),观察组患者PBMC上清液中IL-17水平显著高于对照组(t=3.412,P<0.05)。观察组和对照组患者PBMC中IL-17 mRNA相对表达量分别为4.28±1.34、2.45±0.95,观察组患者PBMC中IL-17 mRNA相对表达量显著高于对照组(t=2.796,P<0.05)。对照组受试者PBMC与观察组患者血清共培养后,随着共培养时间的延长,细胞形态发生明显改变,部分细胞甚至出现细胞膜脱落、崩解、破裂。结论 PBMC中IL-17过度表达可能在MM发生、发展中起一定的促进作用。Objective To investigate the expression of interleukin-17( IL-17) in peripheral blood mononuclear cells( PBMC) of patients with multiple myeloma and analyse it's clinical significance. Methods Thirty patients with multiple myeloma in the Department of Hematopathy,the Third Affiliated Hospital of Xinxiang Medical University from January 2016 to January 2017 were selected as observation group; and thirty healthy donors whose gender and age machted with patients in the observation group were selected as control group. Peripheral blood mononuclear cells were obtained from subjects in observation group and control group by using the method of density gradient centrifugation. The content of IL-17 in supernatant of PBMC culture solution of subjects in the two groups was detected by enzyme linked immunosorbent assay and the expression of IL-17 mRNA in PBMC was detected by quantitative real-time polymerase chain reaction; the cellular morphology of PBMC of subjects in control group was observed by scanning electron microscope after co-culturing with the serum of patients in observation group. Results The content of IL-17 in supernatant of PBMC culture solution of subjects in the observation group and control group was( 30. 79 ± 4. 96),( 10. 10 ± 4. 15) ng·L^(-1) respectively; the content of IL-17 in supernatant of PBMC culture solution of subjects in the observation group was significantly higher than that in the control group( t = 3. 412,P < 0. 05). The expression of IL-17 mRNA in PBMC in the observation group and control group was 4. 28 ± 1. 34 and 2. 45 ± 0. 95 respectively; the expression of IL-17 mRNA in PBMC in the observation was significantly higher than that in the control group( t = 2. 796,P <0. 05). After co-culturing of PBMC of subjects in the control group with the serum of patients in the observation group,the morphology of PBMC changed obviously,and membrane desquamate,membrane disintegration,even membranolysis were observed in some cells with the prolongation of co-culture time. Conclusion The over-ex

关 键 词：多发性骨髓瘤 单个核细胞 白细胞介素-17 

分 类 号：R733.3[医药卫生—肿瘤]