沟纹硬皮地蛛粗毒的多肽成分初探  

A preliminary study on toxin peptides from Calommata signatum crude venom

作　　者：唐兴 陈连水[1] 黄亚洲 段志贵[2] 刘中华[2] 梁宋平[2] 

机构地区：[1]东华理工大学江西省质谱科学与仪器重点实验室,江西南昌330013 [2]湖南师范大学动物多肽药物国家地方联合工程实验室,湖南长沙410081

出　　处：《福建农林大学学报（自然科学版）》2018年第1期104-109,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金项目(31201718;31402025);长江学者和创新团队发展计划项目(IRT13054);江西省自然科学基金项目(20114BAB214015;20142BAB204011);江西省教育厅青年科学基金项目(GJJ12389);东华理工大学博士科研启动基金项目(DHBK1008);湖南省生物发育工程及新产品研发协同中心项目(20134486)

摘　　要：通过高效液相色谱结合MALDI-TOF-TOF质谱对沟纹硬皮地蛛粗毒所含多肽成分进行分离和鉴定.从沟纹硬皮地蛛粗毒中一共鉴定到105种毒素多肽.大多数毒素多肽的分子质量分布范围在1 000~3 500 u(占总数84.8%),还有一部分毒素多肽的分子质量分布范围在3 500~4 500和6 500~8 500 u.与其他蜘蛛种类比较,沟纹硬皮地蛛毒素多肽的分子质量分布特征均不相同,这体现了地蛛科蜘蛛毒液成分的特异性.Peptides of Calommata signatum, crude venom were isolated and identified for the first time by the combination of high performance liquid chromatography and MALDI-T0F-T0F mass spectrometry. Experimental results showed that a total of 105 toxin peptides were identified from C.signatum venom. The majority of toxin peptides fell in mass range of 1 0 0 0 -3 500 u,accounting for 84.8% of the total peptides. Other toxin peptides were in molecular weight of 3 500-4 500 u and 6 5 0 0 -8 500 u. The distribution of molecular weight of venom peptides from C. signatum is ver^^ different from those of other spiders reported,indicating the specificity of venom components from this spider species.

关 键 词：沟纹硬皮地蛛 髙效液相色谱 质谱 毒素多肽 

分 类 号：Q51[生物学—生物化学] Q95