基于PCR-RFLP技术分析H-FABP基因在5个猪群体中的多态性  

Analysis of H-FABP Gene Polymorphisms in Five Pig Groups Based on PCR-RFLP

作　　者：吴华莉[1,2,3] 涂尾龙 曹建国[1,3] 都启晶 常华[5] 羊兆洪 谈永松[1,2,3] 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]上海市调控生物学重点实验室,上海200241 [3]上海种猪工程技术研究中心,上海201302 [4]青岛农业大学食品科学与工程学院,山东青岛266109 [5]云南农业大学动物科学技术学院,云南昆明650201 [6]淮安洪泰良种猪繁育有限公司,江苏淮安223472

出　　处：《西南农业学报》2018年第1期207-210,共4页Southwest China Journal of Agricultural Sciences

基　　金：上海市科委科技支撑项目(16140900200);上海市调控生物学重点实验室开放课题基金资助项目;苏北科技专项(BN2016074)

摘　　要：【目的】肉质是猪肉的重要经济指标,肌内脂肪(IMF)含量是影响肉质的重要因素。H-FABP基因是调控猪肉IMF含量的候选基因。【方法】本实验采用PCR-RFLP技术分析H-FABP基因多态性,并检测该基因在5个猪群体:杜洛克(100头)、长白(80头)、大白(150头)、申农(80头)、梅山(140头)的基因型和基因频率。【结果】H-FABP基因在杜洛克、大白、申农猪群体中检测出DD、Dd和dd 3种基因型。在杜洛克群体中的基因型频率:DD(0.02)、Dd(0.23)和dd(0.75);在大白猪群体中基因型频率DD∶(0.25)、Dd(0.49)和dd(0.26);在申农猪群体中基因型频率:DD(0.10)、Dd(0.30)和dd(0.60)。在长白猪群体中检测的所有样品为dd型;在梅山猪群体中检测的所有样品为DD型。【结论】H-FABP基因在5个猪群体中呈现不同基因型。在长白猪和梅山猪群体中呈现偏态现象。在杜洛克和申农猪群体中主要为dd型,在大白群体中主要为Dd型。【Objective】Meat quality is one of the important indicators of pork. Intramuscular fat content( IMF) is one of the important factors in affecting meat quality. H-FABP gene is a candidate gene as regulating pig intramuscular fat traits. 【Method】The experiment based on PCR-RFLP was conducted,and H-FABP gene polymorphisms in five pig groups: Duroc( 100),Landrace( 80),Large White( 150),Shennong( 80) and Meishan( 140) were detected. 【Result】H-FABP genes in Duroc,Large White and Shennong groups had DD,Dd and dd genotype. The genotype frequencies in Duroc groups were DD( 0. 02),Dd( 0. 23) and dd( 0. 75),the genotype frequencies in Large White groups were DD( 0. 25),Dd( 0. 49) and dd( 0. 26),and the genotype frequencies in Shennong groups were DD( 0. 10),Dd( 0. 30)and dd( 0. 60). The genotype frequencies in Landrace groups were dd( 1. 0),no Dd and DD; Genotype frequencies in Meishan groups: DD( 1. 0). 【Conclusion】H-FABP gene present different genotypes in five pig groups. Only gentype was detected in Landrace and Meishan populations. dd genotype was main genotype in Duroc and shennong groups,Dd genotype was main genotype in Large White groups.

关 键 词：猪 心脏型脂肪酸结合蛋白基因 多态性 

分 类 号：S828[农业科学—畜牧学]