血管紧张素1-7对高脂诱导小鼠肝脏损伤的保护作用  

Protective effect of angiotensin 1-7 on liver injury of mice induce by high fat diet

作　　者：黄文瀚[1] 任飞凤 罗蕾 周俊 潘珠玛 郭晖[3] 唐琳[1] 

机构地区：[1]重庆医科大学附属第二医院风湿免疫科,重庆400010 [2]重庆市第七人民医院内分泌,肾病科,400054 [3]重庆医科大学病毒性肝炎研究所,重庆400016

出　　处：《现代医药卫生》2018年第3期344-345,348,共3页Journal of Modern Medicine & Health

基　　金：国家自然科学基金资助项目(81771738)

摘　　要：目的探讨血管紧张素1-7[Ang-(1-7)]对高脂诱导的小鼠肝脏损伤的保护作用及潜在调节机制。方法将40只健康雄性C57BL/6小鼠随机分为正常组、高脂组、高脂+Ang-(1-7)组、Ang-(1-7)组,建模后分别检测每组小鼠血清中总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白(LDL)的水平,油红O染色检测小鼠肝脏脂质沉积,Western blotting检测肝脏中固醇调节元件结合蛋白(SREBP2)的表达水平。结果高脂组小鼠血清TC、TG、LDL水平均明显高于正常组和高脂+Ang-(1-7)组,差异均有统计学意义(P<0.05);高脂组小鼠肝脏脂质沉积明显,Western blotting提示SREBP2蛋白表达水平因高脂呈负反馈效应,Ang-(1-7)能够增强这一负反馈作用,使SREBP2蛋白表达水平进一步降低,有效阻断机体对脂质的吸收。结论 Ang-(1-7)能够通过调节小鼠SREBP2的表达,从而减轻脂质沉积,发挥保护肝脏作用。Objective To investigate the protective effect and the potential regulatory of angiotensin 1-7 [Ang-(1-7)]on liver injury of mice induced by high fat diet(HFD). Methods Forty healthy male C57 BL/6 mice were randomly dividedinto standard diet group,HFD group,HFD+Ang-(1-7)group and Ang-(1-7)group. The serum total cholesterol(TC),triglyceride(TG)and low density lipoprotein(LDL)in each group were detected after mice modeling was established. The liver lipid depo-sition in mice was observed by oil red O staining,and the expression level of SREBP2 was detected by Western blotting.Results The levels of TC、TG、LDL in the HFD group were obviously higher than those in the standard diet group and the HFD+Ang-(1-7)group,and the difference had statistical significance(P<0.05). The liver lipid deposition in HFD group was evident,theWestern blotting results showed that the expression level of SREBP2 was with negative feedback effect due to high fat,while theAng-(1-7)could increase the negative feedback effect,which further reduce the expression level of SREBP2 and inhibit the ab-sorption of lipid effectively. Conclusion The Ang-(1-7)can inhibit the lipid deposition and protect liver by regulating the ex-pression of SREBP2.

关 键 词：高脂血症 血管紧张素类 肝/损伤 负反馈效应 小鼠 

分 类 号：R575[医药卫生—消化系统]