北极海洋细菌Pseudoalteromonas sp.2018抑菌活性及其代谢产物研究  

Inhibitory Effect of Arctic Marine Bacterium Pseudoalteromonas sp.2018 Against Plant Pathogenic Fungi,and the Study on Its Metabolites

作　　者：何培青 李江[1,2,3] 刘彩云 张停[4,5] 王佩圣 林学政 

机构地区：[1]青岛市海洋天然产物研究开发重点实验室,山东青岛266061 [2]国家海洋局第一海洋研究所,山东青岛266061 [3]国家海洋局海洋生物活性物质重点实验室,山东青岛266061 [4]中国海洋大学医药学院,山东青岛266003 [5]教育部海洋药物重点实验室山东省海洋药物重点实验室,山东青岛266003 [6]青岛市农业科学研究院,山东青岛266100

出　　处：《海洋科学进展》2018年第1期98-107,共10页Advances in Marine Science

基　　金：青岛市基础研究计划项目--具有农用活性的极端微生物及化合物资源库的构建及应用(14-2-4-14-jch);海洋公益性行业科研专项--海藻寡糖设施蔬菜抗病诱导剂开发(201505026-04);国家海洋经济创新发展区域示范资助的重要项目--海洋药源生物种质资源库建设(12PYY001SF08-HYYS-1)

摘　　要：从2014年第六次中国北极科学考察采集的海洋沉积物中,分离获得了具有抑制农作物病原真菌活性的菌株Pseudoalteromonas sp.2018。测定了该菌株对病原真菌的抑制范围和对黄瓜白粉病的大棚防治效果,并对该菌株代谢产物通过硅胶色谱、凝胶色谱及半制备HPLC等进行分离纯化,通过质谱(MS)、氢谱(1 H NMR)和碳谱(13 C NMR)等进行结构鉴定。研究结果表明,Pseudoalteromonas sp.2018菌株对辣椒疫霉(Phytophthora capsici)、褐孢霉(Fulvia fulva)、链格孢(Alternaria alternata)、黄瓜专化型尖孢镰孢(Fusarium oxysporum f.sp.cucumerinum)、番茄专化型尖孢镰孢(Fusarium oxysporumf.sp.lycopersici)和细极链格孢(Alternaria tenuissima)等具有抑制作用,对大棚黄瓜白粉病的防治效果达66.7%;研究分离获得了环(脯-酪)二肽、环(脯-异亮)二肽、环(脯-亮)二肽和环(脯-苯丙)二肽,其中环(脯-酪)二肽和环(脯-亮)二肽对测试病原真菌的最小抑制浓度为125~500μg/mL。该研究为极地微生物农用资源的开发利用提供理论参考。Bacterium strain Pseudoalteromonas sp.2018 was isolated from marine sediment collected during the sixth Chinese Arctic Research Expedition in the year of 2014,and this strain could inhibit the growth of several plant pathogenic fungi.Its antifungal spectrum and control effect on cucumber powdery mildew in a greenhouse were determined.Crude metabolites were extracted from the fermentation broth of the strain and were further separated,and purified copmpounds were obtained using silica gel chromatography,gel chromatography and semi-preparative HPLC chromatography.The structures of these compounds were determined via MS,1 H NMR and 13 C NMR.The results demonstrated that Pseudoalteromonas sp.2018 could inhibit Phytophthora capsici,Fulvia fulva,Alternaria alternata,Fusarium oxysporum f.sp.cucumerinum,Fusarium oxysporumf.sp.lycopersici,Botryosphaeria dothidea,Fusarium sporotrichioides,and Alternaria tenuissima.Its control effect on cucumber powdery mildew in greenhouse reached66.7%.The structures of the metabolites were identified as cyclo(Pro-Tyr),cyclo(Pro-Ile),cyclo(ProLeu),and cyclo(Pro-Phe).The MIC values of cyclo(Pro-Tyr)and cyclo(Pro-Leu)against seven pathogenic fungi were determined as 125~500μg/mL.Our study provides theoretical knowledge for development and utilization of agricultural resources from the polar environment.

关 键 词：北极海洋细菌 抑制农作物病原真菌 环二肽 

分 类 号：Q935[生物学—微生物学]