复方贞术调脂胶囊对兔腹主动脉再狭窄模型血脂及炎症因子的影响  

Effect of Fufang Zhenzhu Tiaozhi capsule on serum lipids and inflammatory factors in rabbit abdominal aortic restenosis model

作　　者：张仁丹[1] 黎土娣 王加伟[1] 邓惠坚 曾智桓[1] ZHANG Ren-dan;LI Tu-di;WANG Jia-wei;DENG Hui-jian;ZENG Zhi-huan(First Affiliated Hospital of Guangdong Pharmaceutical Universit)

机构地区：[1]广东药科大学附属第一医院,广东广州510080

出　　处：《中国病理生理杂志》2018年第2期270-275,共6页Chinese Journal of Pathophysiology

基　　金：广东省科技厅广东省公益研究与能力建设项目(No.2017ZC0212)

摘　　要：目的:通过建立兔腹主动脉粥样硬化球囊成形术后再狭窄模型,探讨复方贞术调脂胶囊(FTZ)对球囊成形术后再狭窄模型动物血脂及炎症因子的影响。方法:将新西兰白兔30只分成5组,除空白对照组外,其余各组均制作腹主动脉内皮剥脱血管狭窄模型。高脂喂养4周后再狭窄模型组及各用药组在血管狭窄部位行经皮球囊扩张成形术制作再狭窄模型。用二维定量冠脉造影工作站对造影结果进行血管狭窄分析,血管造影抽血测定血脂浓度及细胞因子质量浓度,以免疫组化法观察血管中核因子κB(NF-κB)表达情况。结果:血管造影证实,各用药组与再狭窄模型组相比,血管面积狭窄率及直径狭窄率均明显下降(P<0.01)。与再狭窄模型组相比,各用药组血脂浓度及细胞因子质量浓度均显著下降(P<0.05)。免疫组化显示再狭窄模型组中NF-κB表达显著高于空白对照组(P<0.05),各用药组NF-κB均显著减弱(P<0.05)。结论:FTZ可显著降低兔腹主动脉成形术后再狭窄模型的血脂及炎症因子,进而抑制再狭窄,且其抗炎作用可能与调节NF-κB通路、抑制各种炎症因子的产生有关。AIM： To investigate the effect of Fufang Zhenzhu Tiaozhi capsule（ FTZ） on serum lipids and inflammatory factors in rabbits with abdorminal aortic restenosis after balloon angioplasty. METHODS： New Zealand white rabbits（ n = 30） were divided into 5 groups. Except blank control group,the rabbits in other groups were used to establish abdominal aortic endothelium exfoliative vascular stenosis model. After 4 weeks of high-fat diet feeding,the animals in restenosis model group and drug treatment groups underwent percutaneous balloon dilatation in the stenosis. The angiographic stenosis was analyzed by a two-dimensional quantitative coronary angiography workstation with a digital subtraction X-ray machine. Blood samples were taken during angiography and the profiles of serum lipids and cytokines were measured. The expression of nuclear factor-κB（ NF-κB） in the blood vessels was determined by immunohistochemistry. RESULTS： Angiography confirmed that the rates of area stenosis and diameter stenosis were significantly decreased in treatment groups compared with restenosis model group（ P 〈 0. 01）. Compared with restenosis model group,the serum lipid profiles and cytokine concentrations in drug treatment groups were significantly decreased（ P 〈 0. 05）. Immunohistochemistry showed the expression of NF-κB in restenosis model group was significantly higher than that in blank control group and drug treatment groups（ P 〈 0. 05）. CONCLUSION： FTZ significantly reduces the blood lipids and inflammatory factors in abdominal aortic restenosis model,and the anti-inflammatory effect may be related to the regulation of NF-κB pathway to inhibit the production of various inflammatory factors.

关 键 词：复方贞术调脂胶囊 再狭窄 血脂 炎症因子 NF-κB 

分 类 号：R0[医药卫生] R285.5