p62蛋白在结肠癌组织的表达及临床意义  

Expression and clinical significance of p62 protein in colon cancer

作　　者：雷程[1] 葛磊[1] 王琦三[1] 

机构地区：[1]新疆医科大学附属肿瘤医院胃肠外科,乌鲁木齐830011

出　　处：《中华实验外科杂志》2018年第2期204-206,共3页Chinese Journal of Experimental Surgery

基　　金：新疆维吾尔自治区自然科学基金（2017D01C412）

摘　　要：目的探讨p62蛋白在结肠癌组织中的表达及临床意义。方法收集60例散发的结肠癌患者临床资料和手术标本，应用免疫组织化学和Western blot法检测p62蛋白在肿瘤组织和癌旁组织中的表达；应用实时定量反转录聚合酶链反应（RT-qPCR）的方法对标本中p62 mRNA的表达水平进行检测；探讨癌组织中p62的表达水平与患者的临床病理特征之间的相关性。结果60例结肠癌组织与癌旁组织比较，p62 mRNA的表达明显上调（3.179 4±0.817 0比1.386 6±0.448 2），差异有统计学意义（t＝14.633，P＝0.000）；p62蛋白的表达明显上调（0.982 5±0.264 6比0.329 8±0.114 5），差异有统计学意义（t＝20.593，P＝0.000）。p62的表达与患者性别、年龄、民族、肿瘤部位、肿瘤大小、组织学类型和分化程度、肿瘤浸润深度无明显相关，与淋巴结转移及肿瘤分期显著相关（χ^2＝11.250，P＝0.001）。结论p62高表达的结肠癌患者淋巴结转移率高，病期晚；p62的异常累积与结肠癌的发生发展明显相关。Objective To investigate the expression pattern of p62 protein in colon cancer and its clinical significance.Methods Clinical data of 60 patients with sporadic colon cancer were collected, including the gender, age, ethnicity, primary tumor site, tumor size, histological type and degree of differentiation of tumor, tumor invasion depth, lymph node metastasis and TNM stage. The surgical specimens were also collected. The expression of p62 protein in tumor tissues and adjacent normal tissues was detected by immunohistochemistry, real-time reverse transcriptase-polymerase chain reaction（RT-qPCR） and Western blotting, and its correlation with the clinicopathological data were statistically analyzed.Results The expression of p62 mRNA was up-regulated in 60 cases of colon cancer as compared with adjacent tissues （3.179 4±0.817 0 vs. 1.386 6±0.448 2）, and the difference was statistically significant （t=14.633, P=0.000）. As compared with adjacent tissues, the expression of p62 protein was up-regulated in colon cancer tissues （0.982 5±0.264 6 vs. 0.329 8±0.114 5）, and the difference was statistically significant （t=20.593, P=0.000）. The expression of p62 in colon cancer tissue had no significant correlation with the patient’s gender, age, ethnicity, primary tumor site, tumor size, histological type and degree of differentiation of tumor, tumor invasion depth, but correlated to lymph node metastasis and TNM stage （χ^2=11.250, P=0.001）.Conclusion Colon cancer patients with high expression of p62 have higher lymph node metastasis rate and a later disease stage. The abnormal accumulation of p62 may be correlated to the occurrence and development of colon cancer.

关 键 词：P62 结肠癌 免疫组织化学 实时定量反转录聚合酶链反应 WESTERN BLOT 

分 类 号：R735.35[医药卫生—肿瘤]