肺癌肿瘤抑制物1对人大肠癌细胞株HT29增殖与凋亡的影响  

Effect of tumor suppressor in lung cancer 1 gene on proliferation and apoptosis in human colorectal cancer HT29 cells

作　　者：王碧荣 袁高乐 梁启廉 陈国强 李卫东 邓旭斌 王慧 

机构地区：[1]广州医科大学附属肿瘤医院胸部肿瘤科,510095 [2]广东医科大学附属医院肿瘤中心,湛江524001

出　　处：《中华实验外科杂志》2018年第2期234-237,共4页Chinese Journal of Experimental Surgery

基　　金：广州市医药卫生科技一般引导项目（20151A010111）

摘　　要：目的观察肺癌肿瘤抑制物1（TSLC1）对人大肠癌细胞株HT29增殖及凋亡的影响。方法采用反转录-聚合酶链反应（RT-PCR）获得TSLC1全基因片段，构建真核表达载体pIRES2-增强型绿色荧光蛋白（EGFP）-TSLC1并瞬时转染至人大肠癌细胞株HT29，把含pIRES2-EGFP-TSLC1的大肠癌细胞株定义为实验组，含有pIRES2-EGFP的大肠癌细胞株定义为对照组，空白大肠癌细胞株定义为空白对照组，通过噻唑蓝（MTT）法测定3种细胞株增殖，流式细胞仪测定细胞周期变化，采用膜联蛋白V（Annexin V）/碘化丙锭（PI）双染法测定3组细胞的凋亡。结果成功构建TSLC1真核表达载体；实验组细胞可抑制细胞增殖[G1期：（82.55±2.71）%，Q＝20.873，P＝0.000；S期：（10.36±1.84）%，Q＝10.003，P＝0.001；G2/M期：（7.09±1.32）%，Q＝14.418，P＝0.000]，促进细胞凋亡[（11.98±1.12）%，Q＝22.096，P＝0.000]。结论抑癌基因TSLC1对人大肠癌细胞株HT29有明显的抑制生长、促进凋亡作用。Objective To probe the effects of tumor suppressor in lung cancer 1 （TSLC1） on proliferation and apoptosis in human colorectal cancer HT29 cells.Methods The intact gene fragments of TSCL1 was acquired by reverse transcriptase-polymerase chain reaction （RT-PCR）, and the eukaryotic expression vector with TSLC1 gene [pIRES2-enhanced green fluorescent protein （EGFP）-TSLC1] was constructed to transient transfection in colorectal cancer HT29 cells. The cells which were transfected eukaryotic expression vector with TSLC1 gene （pIRES2-EGFP-TSLC1） were defined as experimental group; the cells which were ransfected eukaryotic expression vector （pIRES2-EGFP） were defined as control group; colorectal cancer HT29 cells were defined as blank control group. The proliferation of three cells was measured by methyl thiazol tetrazolium （MTT） assay; the cell cycle was measured with flow cytometry （FCM）; cell apoptosis was measured by Annexin-V/propidium iodide （PI） double staining FCM.Results The eukaryotic expression vector with TSLC1 gene （pIRES2-EGFP-TSLC1） was constructed integrallty. Experimental group inhibited cell proliferation [G1 phase： （82.55±2.71）%, Q=20.873, P=0.000; S phase： （10.36±1.84）%, Q=10.003, P=0.001; G2/M phase： （7.09±1.32）%, Q=14.418, P=0.000] and promoted apoptosis [（11.98±1.12）%, Q=22.096, P=0.000].Conclusion The TSLC1 gene had an obvious inhibition of growth and accelerating apoptosis in colorectal cancer HT29 cells. The results of this study established experimental foundation to further study on the mechanism of TSLC1 in colorectal cancer.

关 键 词：肺癌肿瘤抑制物1 大肠癌细胞 凋亡 细胞周期 

分 类 号：R735.34[医药卫生—肿瘤]