微小RNA-151-5p在甲状腺乳头状癌组织的表达及在淋巴结转移中的意义  

Expression of microRNA - 151 - 5p in papillary thyroid carcinoma and its significance in lymph node metastasis

作　　者：柯建波 凌志强[3] 王文栋[2] 聂喜林[2] 王可敬[2] 顾佳磊[2] 赏金标 

机构地区：[1]浙江中医药大学第二临床医学院,杭州310053 [2]浙江省肿瘤医院头颈外科,杭州310022 [3]浙江省肿瘤研究所

出　　处：《中华实验外科杂志》2018年第2期328-330,共3页Chinese Journal of Experimental Surgery

基　　金：浙江省自然科学基金面上项目（LY15H160008）

摘　　要：目的观察微小RNA（miRNA，miR）-151-5p和miR-151-3p在甲状腺乳头状癌（PTC）中的表达并探讨其临床意义。方法采用实时定量聚合酶链反应（Real-time PCR）检测200例PTC组织及配对癌旁组织中miR-151-5p和miR-151-3p表达水平，探讨miR-151-5p和miR-151-3p表达与PTC患者临床病理特征的关系。结果miR-151-5p在PTC的相对表达量（8.49±1.18）显著高于癌旁组织（1.91±0.26, P＝0.000），有淋巴结转移的PTC组织中miR-151-5p相对表达量（10.35±1.36）显著高于无淋巴结转移者（5.98±1.25，P＝0.034）。miR-151-3p在PTC的相对表达量（9.25±1.26）显著高于癌旁组织（2.02±0.31，P＝0.000），但PTC转移组（9.41±1.29）与非转移组（9.04±1.23）比较miR-151-3p表达差异无统计学意义（P＝0.658）。miR-151-5p高表达与PTC患者性别（P＝0.000）、病灶数量（P＝0.004）、局部侵犯（P＝0.018）、淋巴结转移（P＝0.000）和病理分期（P＝0.000）显著相关。结论miR-151-5p在PTC组织中表达上调，与淋巴结转移明显相关。Objective To observe the expression of microRNA （miRNA, miR）-151-5p and miR-151-3p in papillary thyroid carcinoma （PTC） and explore its clinical significance.Methods The levels of miR-151-5p and miR-151-3p expression in 200 cases of PTC tissues and paired normal tissues were detected by real-time quantitative polymerase chain reaction （Real-time PCR）. The relationship between the expression of miR-151-5p and miR-151-3p and the clinicopathological characteristics of PTC patients was investigated.Results The level of miR-151-5p expression in PTC tissues （8.49±1.18） was significantly higher than that in adjacent normal tissues （1.91±0.26, P=0.000）. The level of miR-151-5p expression in PTC patients with lymph node metastasis （10.35±1.36） was significantly higher than that in those without lymph node metastasis （5.98±1.25, P=0.034）. The level of miR-151-3p expression in PTC tissues （9.25±1.26） was significantly higher than that in adjacent normal tissues （2.02±0.31, P=0.000）. However, there was no significant difference in miR-151-3p expression level between PTC patients with lymph node metastasis （9.41±1.29） and those without lymph node metastasis （9.04±1.23, P=0.658）. The high expression of miR-151-5p was significantly correlated with gender （P=0.000）, number of lesions （P=0.004）, local invasion （P=0.018）, lymph node metastasis （P=0.000） and clinical stages （P=0.000） in PTC patients.Conclusion MiR-151-5p is significantly up-regulated in PTC tissues and closely related to lymph node metastasis.

关 键 词：甲状腺乳头状癌 微小RNA-151-5p 微小RNA-151-3p 淋巴结转移 

分 类 号：R736.1[医药卫生—肿瘤]