改进的大鼠右室流出道梗阻动物模型的建立  

Establishment of modified right ventrtcular outflow tract obstruction animal model In rats

作　　者：陈少钦 马军 陈良万[1] 方冠华[1] 廖东山[1] 黄秋瑜 

机构地区：[1]福建医科大学附属协和医院心血管外科,福州350001

出　　处：《中华实验外科杂志》2018年第2期357-359,共3页Chinese Journal of Experimental Surgery

摘　　要：目的改进右室流出道梗阻动物模型制作方法，探讨更简便易行及符合临床病理过程的模型制作方法。方法30只SD雄性大鼠随机分为手术组（RVOTO）、假手术组、对照组。每组10只。手术组用U型夹施行肺动脉主干缩窄术，假手术组开胸但不进行肺动脉主干缩窄，对照组不施行外科操作。4周后收集心脏标本，测量右室厚度和心肌细胞纤维容积比。利用t检验对数据进行分析比较。结果手术组和对照组、假手术组对比，右室心肌明显增生肥厚，右室平均厚度[手术组：（0.938±0.054） mm；假手术组：（0.260±0.436） mm；对照组：（0.249±0.587） mm，P＝0.000]，尤其以右室流出道肥厚为主，Masson染色显示右室心肌肉细胞肥大紊乱，纤维化明显。手术组（20.17±5.87）%，假手术组（8.35±4.23）%，对照组（7.57±2.31）%（P＝0.000）。结论改进的右室流出道梗阻动物模型，成本低廉，简便易行，创伤小，手术成功率高，更符合临床病理变化。Objective To modify the model of right ventricular outflow tract obstruction （RVOTO） in rats, and to search for the optimal method for the model establishment.Methods Thirty male SD rats were randomly divided to three groups： RVOTO group, sham group and control group. There were 10 rats in each group. The animals in RVOTO group were given pulmonary arterial constriction （PAC）, those in sham group thoracotomy without PAC, and those in control group no surgical operation. The heart specimens were harvested after 4 weeks. The thickness of right ventricle, the hyperplastic state of myocardiac cells and the fiber volume ratio of myocardiac cells were analyzed by the t test.Results The modified RVOTO model was established in 4 weeks. The thickness of right ventricle in RVOTO group was greater than that in sham group and control group [RVOTO group： （0.938±0.054） mm; sham group： （0.260±0.436） mm; control group： （0.249±0.587） mm, P=0.000]. The thickness of right ventricular outflow tract was significantly increased as compared with the free wall of right ventricle. The fiber volume ratio of myocardiac cells in RVOTO group was significantly higher than in sham group and control group [RVOTO group： （20.17±5.87）%; sham group： （8.35±4.23）%; control group： （7.57±2.31）%, P=0.000].Conclusion The modified model of RVOTO in rats is significantly cheaper, easier, safer and more consistent with the clinical features than the traditional one.

关 键 词：右室流出道梗阻 右室肥厚 U型夹 模型 动物 

分 类 号：R-332[医药卫生]