miR141对前列腺癌LNCaP细胞周期的影响及其机制研究  

Effects of miR141 on cell cycle in LNCa P human prostate cancer cells and its mechanism

作　　者：杨森[1] 范来来 顾希杰 柯润疆 木海琦[1] 王怡君[1] 南存金[1] 陈映鹤[1] YANG Sen;FAN Lai-lai;GU Xi-jie;et al(Department of Urology Surgery, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou , Zhejiang 325000, Chin)

机构地区：[1]温州医科大学附属第二医院泌尿外科,浙江温州325000

出　　处：《中华全科医学》2018年第1期22-25,63,共5页Chinese Journal of General Practice

基　　金：2015年浙江省自然科学基金(LY15H050007);2017年浙江省温州市科技局公益性社会发展科技项目(Y20170100)

摘　　要：目的观察miR141对LNCa P细胞的增殖及其细胞周期的影响。方法收集温州医科大学附属第二医院2015年12月—2017年6月前列腺癌患者癌组织及癌旁组织,采用q PCR检测miR141表达情况;同时检测LNCa P细胞miR141表达情况。将LNCa P细胞分为LNCa P组(不进行转染)、mimics组(转染miR141-mimics)与inhibitor组(转染miRNA-141 inhibitor)。观察其转染效率,并用MTT法检测3组细胞的增殖率,流式细胞仪检测3组细胞的细胞周期情况,通过WB检测P27、CDK4和Cyclin D1表达情况。结果在癌组织中miR-141的水平显著高于癌旁组织(P<0.05),在LNCa P细胞中miRNA-141的水平相比于癌组织较低(P<0.05),但略高于癌旁组织。LNCa P细胞转染miRNA-141-mimics后,miRNA-141表达显著升高(P<0.05),转染miRNA-141-mimics及miRNA-141 inhibitor后,miRNA-141表达略高于LNCa P细胞。在LNCa P转染miRNA-141 mimics 48 h时,细胞增殖率相比于LNCa P及inhibitor组要低,说明miRNA-141能阻碍LNCa P细胞的增殖。在LNCa P转染miRNA-141 mimics后,细胞周期在G_1期发生阻滞。mimics组与LNCa P组、inhibitor组相比,P27明显升高,而CDK4和Cyclin D1则明显下降。结论 miR141影响LNCa P的增殖,能使LNCa P阻滞在G_1期。Objective To observe the effect of miR141 on the proliferation and cell cycle in LNCaP human prostate canc- er cells. Methods The cancer tissues and adjacent tissues of patients with prostate cancer in our hospital from Decem- ber, 2015 to June, 2017 were collected, and the miR141 expression were detected by using qPCR. The expression of miR141 in LNCaP cell was also detected. LNCaP cells were divided into LNCaP group （without transfection） , mimics group （transfected with miR141-mimics） and inhibitor group （transfected with miRNA-141 inhibitor） to observe its transfection efficiency. The increment rate of the three groups was detected by MTF method, and the cell cycle was detec- ted by flow cytometry. The expression of P27, CDK4 and CyclinD1 were detected by WB. Results The levels of the miRNA-141 in cancer tissues were significantly higher than that of the adjacent tissues （P 〈 0.05 ） , and the levels of the miRNA-141 in the LNCaP cells were lower than those of the cancer tissues （P 〈 0.05 ）, but slightly higher than those of the adjacent tissues. After LNCaP ceils transfected with miRNA-141-mimics, miRNA-141 expression was significantly in- creased （ P 〈 0.05 ）. After the LNCaP cells were transfected, miRNA-141 expression of mimics group and inhibitor group were slightly higher than that of LNCaP group. When LNCaP transfected miRNA-141 mimics 48 h, the cell proliferation rate of mimics group was lower than LNCaP and inhibitor group. After LNCaP transfected miRNA-141 mimics, cell cycle was blocked in G1 phase. Compared with LNCaP group and inhibitor group, the mimics group significantly increased P27, while CDK4 and CyclinD1 decreased significantly. Conclusion MiR141 can affect LNCaP proliferation and block LNCaP in G1 phase.

关 键 词：微小核糖核酸141 前列腺癌 细胞增殖 细胞周期 

分 类 号：R737.25[医药卫生—肿瘤] R730.23[医药卫生—临床医学]