小鼠肝硬化—肝癌上皮间质转化的研究  

Study of the Epithelial Mesenchymal Transition between Liver Cirrhosis and Liver Cancer in Mice

作　　者：孙洪利[1] 

机构地区：[1]中国医科大学附属盛京医院胆道血管外科,沈阳110004

出　　处：《中国医科大学学报》2018年第2期157-161,共5页Journal of China Medical University

基　　金：沈阳市科学技术计划(F14-158-9-48)

摘　　要：目的通过建立动物模型,观察肝硬化及肝癌上皮间质转化过程。方法建立小鼠肝硬化、肝癌动物模型,进行HE染色和免疫组化染色。结果存活小鼠肝硬化诱导成功率100%,病死率30%。存活小鼠肝癌诱导成功率100%,病死率40%。在肝硬化和肝癌动物模型中,E-钙黏蛋白阳性表达率为46.7%和7.1%,β3整联蛋白阳性表达率为40.0%和7.1%,波形蛋白阳性表达率为13.6%和40.0%,纤维连接蛋白阳性表达率为20.0%和57.1%。结论小鼠肝硬化和肝癌组织模型中,E-钙黏蛋白、β3整联蛋白的低表达和波形蛋白、纤维连接蛋白的高表达与肝硬化向肝癌的上皮间质转化过程有关,为临床检测的重要参照指标。Objective To establish animal models facilitating observation of the process of epithelial-mesenchymal transition between liver cirrhosis and liver cancer. Methods Liver cirrhosis and liver cancer models were established in mouse and subsequently assessed using HE and immunohistochemical staining. Results The success rate of the liver cirrhosis model was 100% and the fatality rate was 30%. The success rate of the liver cancer model was also 100% and the fatality rate was 40%. In the liver cirrhosis and liver cancer mod- els, the positive expression rates were, respectively, 46.7% and 7.1% for E-cadherin, 40.0% and 7.1% for β 3-integrin, 13.6% and 40.0% for vimentin, and 20.0% and 57.1% for fibroneetin. Conclusion In mouse models of liver cirrhosis and liver cancer, low expression lev- els of E-cadherin and β 3-integrin in addition to high expression levels of fibronectin and vimentin are related to the epithelial-mesenehy- mal transition between liver cancer and cirrhosis, and may be important indicators for clinical detection.

关 键 词：肝硬化模型 肝癌模型 E-钙黏蛋白 β 3整联蛋白 波形蛋白 纤维连接蛋白 上皮间质转化 

分 类 号：R735.7[医药卫生—肿瘤]