miR-144-3p分子在胶质瘤中的表达  

Transcription of miR-144-3p in glioma and the significance

作　　者：姬丽娅 马妮 王新来[2] 狄政莉[2] 史明 李三中 吴中亮 

机构地区：[1]空军军医大学西京医院神经内科,陕西西安710032 [2]西安市中心医院神经内科,陕西西安710003 [3]空军军医大学西京医院神经外科,陕西西安710032

出　　处：《中华神经外科疾病研究杂志》2018年第1期9-12,共4页Chinese Journal of Neurosurgical Disease Research

摘　　要：目的本文研究了小分子核糖核酸-144-3p(miR-144-3p)分子与胶质瘤发生发展的相关性。方法本实验所需胶质瘤组织均来自于西京医院神经外科胶质瘤患者,共50例;利用人源神经元细胞系HCN-2(human cortical neuron-2,HCN-2)、U251胶质瘤细胞系、U87MG胶质瘤细胞系等。通过细胞培养及miRNA转染,细胞及组织RNA提取,反转录多聚酶链式反应(polymerase chain reaction,PCR),实时定量PCR对miR-144-3p分子在胶质瘤中的表达进行检测。结果 miR-144-3p分子在人和小鼠染色质定位高度保守;miR-144-3p分子在胶质瘤患者不同组织中的表达差异;miR-144-3p分子在不同病理学分级胶质瘤中的表达差异;miR-144-3p分子在不同人源胶质瘤细胞系中的表达差异,其表达量与患者预后相关性。结论 miR-144-3p分子在哺乳动物中高度保守,与患者的预后有关。Objective The relationship between miR-144-3p and the development and prognosis of glioma were studied. Methods A total of 50 tissue samples of glioma with different cell types and grades were obtained from patients in Department of Neurosurgery of Xijing Hospital. Different cell lines of human gliorma （human cortical neuron-2, HCN-2, U251, UgTMG） were cultured and the cells were collected, miR-144-3p in the tissue samples and cell lines were isolated and the levels of miR-144-3p were detected by quantitative reverse-transcription polymerase chain reaction （PCR）. Results The results revealed that miR-144-3p was differentially expressed in normal tissues and glionm tissues. There was a negative correlation between the expression levels of miR-144-3p and the severities of grades of glioma. Moreover, the levels of miR-144-3p were positively related to the survival days in patients with glioma. Conclusion MiR-ld4-3p is highly conserved in mammalian. It troy be used as an indicator to the prognosis of patients with glioma.

关 键 词：胶质瘤 小分子核糖核酸-144-3p 多聚酶链式反应 

分 类 号：R739[医药卫生—肿瘤]