子宫内膜癌患者病灶超声造影成像检查结果、增殖凋亡基因编码蛋白表达及其相关性分析  

Results of ultrasound contrast imaging of patients with endometrial cancer,expression of proliferation and apoptosis gene encoded proteins and the relationships between them

作　　者：回亚男[1] 郝桂纯[1] 杨静[1] 史艺[2] 

机构地区：[1]天津中医药大学第一附属医院,天津300193 [2]天津市第三中心医院

出　　处：《山东医药》2018年第3期19-22,共4页Shandong Medical Journal

基　　金：天津市卫生局医药卫生科技计划项目(2016KZ49)

摘　　要：目的探讨子宫内膜癌病灶超声造影参数、病灶组织增殖凋亡蛋白表达变化及其相关性。方法选择79例子宫内膜癌患者为子宫内膜癌组,53例子宫肌瘤患者为子宫肌瘤组。两组患者均行超声造影成像检查,记录病灶峰值强度(Peak)、达峰时间(TTP)和造影剂平均渡越时间(MMT)。均行子宫切除术,留取病灶组织,采用Western blotting法测算两组病灶组织Survivin、Bcl-2、Bax、Beclin1、CDK4、CDK6蛋白相对表达量。结果子宫内膜癌组病灶Peak、MTT、TTP均高于子宫肌瘤组病灶(P均<0.05)。子宫内膜癌组病灶组织Survivin、Bcl-2、CDK4、CDK6表达量高于子宫肌瘤组(P均<0.05),Bax和Beclin1蛋白表达量低于子宫肌瘤组(P均<0.05)。子宫内膜癌患者病灶Peak与病灶组织Survivin、Bcl-2、CDK4、CDK6表达量均呈正相关(r分别为0.704、0.692、0.737、0.730,P均<0.05),与Bax和Beclin1蛋白表达量均呈负相关(r分别为-0.687、-0.709,P均<0.05);MTT与病灶组织Survivin、Bcl-2、CDK4、CDK6表达量均呈正相关(r分别为0.689、0.731、0.725、0.694,P均<0.05),与Bax和Beclin1蛋白表达量均呈负相关(r分别为-0.714、-0.698,P均<0.05);TTP与病灶组织Survivin、Bcl-2、CDK4、CDK6表达量均呈负相关(r分别为-0.706、-0.725、-0.723、-0.701,P均<0.05),与Bax和Beclin1蛋白表达量均呈正相关(r分别为-0.696、-0.719,P均<0.05)。结论与子宫肌瘤患者相比,子宫内膜癌患者病灶超声造影参数Peak、MTT、TTP较高,病灶组织Survivin、Bcl-2、CDK4、CDK6表达量较高,Bax、Beclin1表达量较低。子宫内膜癌患者病灶Peak、MTT、TTP与病灶组织增殖、凋亡蛋白Survivin、Bcl-2、Bax、Beclin1、CDK4、CDK6表达量有关。对子宫内膜癌患者病灶行超声造影成像检查有助于判断患者细胞凋亡相关蛋白表达情况。Objective To explore the ultrasonography parameters of endometrial carcinoma（ EC） and the expression changes of prolifer-ation and apoptosis gene encoded proteins in the lesion tissues as well as the relationship between them.Methods Seventy-nine cases of patients with EC were selected as the EC group,and 53 cases of patients with hysteromyoma were treated as the hysteromyoma group. The patients of the two groups were examined by ultrasound contrast imaging. The peak intensity of the lesion（ Peak）,peak time（ TTP）,and the average transit time of contrast agent（ MMT）were recorded. The hysterectomy was performed to obtain the lesion tissues. Western blotting was used to measure the relative expression of Survivin,Bcl-2,Bax,Beclin1,CDK4,and CDK6 proteins in the two groups. Results The Peak,MTT,and TTP of the EC group were higher than those of the hysteromyoma group（ all P〈0. 05）. The expression levels of Survivin,Bcl-2,CDK4,and CDK6 in the EC group were higher,while the expression levels of Bax and Beclin1 proteins were lower than those in the hysteromyoma group（ all P〈0. 05）. The Peak was positively correlated with Survivin,Bcl-2,CDK4,and CDK6 in the EC patients（ r = 0. 704,0. 692,0. 737,and 0. 730; all P〈0. 05）,and was negatively correlated with Bax and Beclin12 proteins（ r =-0. 687 and-0. 709,both P〈0. 05）. MTT was positively correlated with the expression of Survivin,Bcl-2,CDK4,and CDK6 in the lesion tissues（ r = 0. 689,0. 731,0. 725,and 0. 694,all P〈0. 05）,and was negatively correlated with the expression of Bax and Beclin12 proteins（ r =-0. 714 and-0. 698,both P〈0. 05）. TTP was negatively correlated with expression of Survivin,Bcl-2,CDK4,and CDK6 in the lesion tissues（ r =-0. 706,-0. 725,-0. 723,and-0. 701; all P〈0. 05）,and was positively correlated with the expression of Bax and Beclin12 proteins（ r =-0. 696 and-0. 719,both P〈0. 05）. Conclusions Compared with the patients with hysteromyoma,the ultrasound contrast parameters Peak,MTT,and TTP are hi

关 键 词：超声造影成像 细胞凋亡 增殖凋亡基因 子宫内膜癌 

分 类 号：R730.4[医药卫生—肿瘤]