NaCl胁迫对耐盐性不同葡萄株系叶片活性氧代谢及清除系统的影响  

Effects of Salt Stress on the Generation and Scavenging of Reactive Oxygen Species in Leaves of Grape Strains with Different Salt Tolerance

作　　者：付晴晴[1] 谭雅中 翟衡[1] 杜远鹏[1] 

机构地区：[1]山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,农业部黄淮地区园艺作物生物学与种质创制重点实验室,山东泰安271018

出　　处：《园艺学报》2018年第1期30-40,共11页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31572084); 国家现代农业产业技术体系建设专项资金项目(CARS-29); 长江学者和创新团队发展计划项目(IRT15R42); 山东省公益性重点研发计划项目(2017GNC13112)

摘　　要：探究耐盐性不同的葡萄株系叶片活性氧产生及其清除系统对盐胁迫的响应,以期揭示耐盐性较强的葡萄种间杂交F_1代株系的耐盐机制。采用组培苗营养液培养方式,以山葡萄‘左山一’与砧木SO4杂交初选的耐盐性较强的F_1代株系(A15、A17)为试材,以1103P为参照品种,进行100 mmol·L^(-1)NaCl处理,测定各株系叶片的O_2^-产生速率、H_2O_2含量、相对电导率、MDA含量和叶绿素含量以及抗氧化酶活性和抗氧化物质含量等指标,并观察叶绿体超微结构。结果表明,100 mmol·L^(-1 )Na Cl胁迫下,耐盐性较强的A15和A17能够维持较高的SOD、CAT、POD等抗氧化酶的活性,保持AsA-GSH循环系统中较高的AsA和GSH含量以及APX、GR等酶的活性,ROS、MDA产生较少,相对电导率升高幅度较小,叶绿体结构较完整,维持较高的叶绿体色素含量;而耐盐性较弱的1103P体内产生大量的ROS和MDA,叶绿体膜结构的完整性遭到破坏,叶绿素含量下降幅度大。In order to study the generation and scavenging of reactive oxygen species（ROS）in leaves of grape strains with different salt tolerance under salt stress,the salt-tolerance tissue culture hybrids（A15 and A17）of Vitis amurensis Rupr.‘Zuoshan 1’×SO4 were used as materials to be irrigated with 100mmol·L^（-1 ）Na Cl,and tissue culture 1103P was used as negative control.production rate,H_2O_2 content,MDA content,relative electrical conductivity,chlorophyll content,antioxidant enzyme activities and antioxidant substances were determined.The results showed that,under salt stress,SOD,CAT and POD activities,As A and GSH content and APX and GR activities in leaves were higher in salt-tolerancehybrids A15 and A17,ROS and MDA content were lower,and the increment of relative electrical conductivity was less,the structure of chloroplast was integrated,and the chloroplast content was higher;while the overproduction of ROS and MDA damaged the membrane integrity and reduced chloroplast content in 1103P.Salt-tolerance hybrids A15 and A17 remained high antioxidase activity and As A-GSHcycle efficiency,reduced the damage of membrane caused by lipid peroxidation,maintained the structure and function integrity of chloroplast.

关 键 词：葡萄 砧木 杂交株系 NACL胁迫 叶片 活性氧代谢 

分 类 号：S663.1[农业科学—果树学]