紫甘薯花色苷对膀胱癌BIU87细胞增殖的影响及其机制探讨  

The influence and mechanisms of purple sweet potato anthocyanins on the growth of bladder cancerBIU87 cell

作　　者：李卫林 季广华 章新展 俞洪元 

机构地区：[1]浙江省温州医科大学附属台州医院泌尿外科,浙江台州317000

出　　处：《中华医学杂志》2018年第6期457-459,共3页National Medical Journal of China

基　　金：浙江省中医药科学研究基金项目（2014ZB135）

摘　　要：目的观察紫甘薯花色苷对膀胱癌BIU87细胞增殖的影响并探讨其机制。方法从紫甘薯中提取花色苷的浓缩液，以不同浓度（100、200、400、800μg/m1）的花色苷干预BIU87细胞48h，通过细胞形态学观察以及细胞计数试剂盒（CCK-8法）检测花色苷对BIU87细胞的增殖抑制作用，流式细胞术（FCM）检测细胞凋亡率。结果形态学表现为随着花色苷浓度的升高BIU87细胞数量减少、细胞体积缩小、细胞间隙增大、细胞黏附性变差、细胞形态变形。CCK-8法检测显示不同浓度的花色苷对BIU87细胞均有显著的增殖抑制作用，100、200、400、800μg／ml的花色苷处理BIU87细胞48h的吸光度（A）值分别为1．24±0．07、1．15±0．11、0．90±0．08、0．56±0．09，与对照组相比，差异均有统计学意义（均P〈0．05）。FCM结果显示，100、200、400、800μg／ml花色苷浓度组的凋亡率分别为7．31％、11．11％、25．96％、36．28％，随着花色苷浓度的升高，凋亡率逐渐增大。结论紫甘薯花色苷可通过诱导细胞凋亡抑制膀胱癌BIU87细胞的增殖，且具有剂量一依赖性。Objective To observe the effect of purple sweet potato anthocyanins on the proliferation of bladder cancer cell line BIU87 and to investigate the molecular mechanisms. Methods Bladder cancer BIU87 cells were cultured and exposed to anthocyanins at the different concentrations of 100,200,400, and 800 μg/ml respectively. The growth inhibition of anthocyanins on BIU87 cells were evaluated by morphometry and cell counting kit-8 （ CCK-8 ） assay, and the cell apoptosis rate was detected by Flow cytometry （FCM）. Results Morphometry showed that the number of BIU87 cells decreased, the volume shrank, the intercellular space enlarged, the ability of cell adherence weakened, and the cell shape changed when the concentration of anthocyanins increased. CCK-8 assay showed that when 100,200,400, 800 μg/ ml anthocyanins treated BIU87 ceils for48 h, the absorbance was 24 ± 0.07, 1.15 ± 0. 11, 0.90 ± O. 08, 0. 56 ± 0. 09, respectively. Compared with the control group, anthocyanins-treated groups significantly inhibited the proliferation of BIU87 cells （P 〈 0. 05）. FCM test showed that after treatment with different doses of anthocyanins, the apoptosis rate was 7.31% , 11.11%, 25.96% , 36. 28%, respectively, and with the concentration of anthocyanins being higher, the apoptosis rate of BIU87 cells was being higher. Conclusion Purple sweet potato anthocyanins can inhibit the growth of bladder cancer BIU87 cells through inducing cell apoptosis in a dose-dependent manner.

关 键 词：紫甘薯花色苷 膀胱癌细胞 凋亡 

分 类 号：R285[医药卫生—中药学]