PI3K信号通路中LCK、LAT、SYK、PKC基因在特发性眼眶炎性假瘤中差异表达分析  

Analysis of differential expression LCK、LAT、SYK and PKC in PI3K signaling pathway of idiopathic orbital inflammatory pseudotumor

作　　者：李金茹[1] 马建民 李静[1] 王霄娜[1] 

机构地区：[1]首都医科大学附属北京同仁医院眼科,北京100000

出　　处：《贵州医药》2018年第2期137-139,F0003,共4页Guizhou Medical Journal

基　　金：国家自然科学基金(81170875;81371052);北京市卫生系统高层次卫生技术人才培养计划课题(2011-3-041)

摘　　要：目的分析特发性眼眶炎性假瘤患者与单纯眼眶海绵状血管瘤患者病变泪腺标本组织中LCK、LAT、SYK、PKC的差异表达情况,为特发性眼眶炎性假瘤发病机理提供参考。方法采用全基因组表达谱芯片技术检测特发性眼眶炎性假瘤患者病变泪腺组织与单纯眼眶海绵状血管瘤患者瘤体组织中信号通路的差异性基因表达。采用PCR扩增技术,免疫组织化学染色方法及Western blot法检测特发性眼眶炎性假瘤患者病变泪腺组织中LCK、LAT、SYK、PKC的mRNA及蛋白质表达情况,并以单纯眼眶海绵状血管瘤患者瘤体组织标本作对照。结果经基因芯片检测分析,实验组与对照组相比较,PI3K信号通路(PI3Ksignaling pathway)表达显著上调,其中LCK、LAT、SYK、PKC 4个基因差异性表达最为明显(P<0.05)。经PCR扩增技术检测、免疫组织化学方法及Western blot检测,IOIP组LCK、LAT、SYK、PKC的mRNA及蛋白表达均呈阳性,眼眶海绵状血管瘤组表达均呈阴性。结论PI3K信号通路中LCK、LAT、SYK、PKC因子高表达在眼眶特发性炎性假瘤的发生发展中起重要作用。Objective To analyze the differential expression of LCK、LAT、SYK and PKC in lacrimal gland specimens of patients with idiopathic orbital inflammatory pseudotumor or simple orbital cavernous hemangioma,and provide reference for the pathogenesis of idiopathic orbital inflammatory pseudotumor.Methods Different expression of pathways on patients with Idiopathic Orbital Inflammatory Pseudotumor and orbital cavernous hemangioma were detected by gene chips.The expression of LCK、LAT、SYK、PKC in level of mRNA and protein were detected by PCR,immune histochemical exam and western blot in Idiopathic Orbital Inflammatory Pseudotumor and orbital cavernous hemangioma.Orbital cavernous hemangioma was considered as control group.Results Compared with control group,the experimental group was found that the expression of PI3 Ksignaling pathway increased and there were LCK、LAT、SYK、PKC expressed differently.Stripes of DNA amplification were founded in LCK、LAT、SYK、PKC while no stripe was showed in RASA1 in Idiopathic Orbital Inflammatory Pseudotumor.There was no stripe of DNA amplification in orbital cavernous hemangioma.LCK、LAT、SYK、PKC were positive expression in Idiopathic Orbital Inflammatory Pseudotumor.The expression of LCK、LAT、SYK、PKC was normal in orbital cavernous hemangioma.Positive stripes were showed in LCK、LAT、SYK、PKC Idiopathic Orbital Inflammatory Pseudotumor.There was no stripe in orbital cavernous hemangioma.Conclusion Over-expression of LCK、LAT、SYK、PKC in PI3 K signaling pathwaymay take part in the pathogensis of Idiopathic Orbital Inflammatory Pseudotumor.

关 键 词：特发性眼眶炎性假瘤患者 发病机制 PI3K信号通路 泪腺 

分 类 号：R739.72[医药卫生—肿瘤]