蚕蛹蛋白替代蛋白胨研制新型细菌培养基的初步研究  

Preliminary Research in Preparation of New Bacterial Medium by Using Silkworm Pupa Protein Instead of Peptone

作　　者：刘洋 左振宇 石应荣 李梦婷 李广路 

机构地区：[1]武汉科技大学化学与化工学院,湖北武汉430081

出　　处：《化学与生物工程》2018年第2期43-48,共6页Chemistry & Bioengineering

基　　金：大学生科技创新基金项目(16ZRA043)

摘　　要：为了探究蚕蛹蛋白在细菌培养方面的能力及替代蛋白胨配制细菌培养基的可行性,将蚕蛹预处理后经酶解消化得到可溶性蚕蛹蛋白粉,将其以不同比例替代或完全替代普通LB培养基中的蛋白胨成分进行革兰氏阳性菌枯草芽孢杆菌CGMCC1.3358和阴性菌大肠杆菌AB90054的培养实验。结果表明:蚕蛹经酸洗、脱脂脱色、酶解制备可溶性蚕蛹蛋白粉的收率为48.3%,蛋白复溶率可达99.4%;蚕蛹蛋白培养基经高压蒸汽灭菌后色泽微黄且清亮;蚕蛹蛋白可完全替代蛋白胨提供细菌生长所需的氮源和碳源;蚕蛹蛋白培养基能在保证菌体生物量快速积累的同时维持菌体生长形态正常。蚕蛹蛋白具有来源广、价格低、可工业化加工等特点,可作为一种新的廉价的蛋白胨替代原料进行新型细菌培养基的研制。In order to explore the ability of silkworm pupaprotein in bacterial cultivation and feasibility of bacterial medium preparation instead of peptone,we prepared soluble silkworm pupaprotein powder by pretreatment and controlled enzymatic digestion.We conducted the bacterial culture experiment of Gram positive bacteriumBacillus subtilis CGMCC1.3358 and Gram negative bacterium Escherichia coli AB90054 in a new bacterial medium,in which silkworm pupaprotein powder with different proportions instead of peptone in common LB medium.The results show that the yield of soluble silkworm pupaprotein powder reaches 48.3% after pickling,degrease,decolorization,and enzymatic digestion,and the re-solubility of the protein reaches 99.4%.The silkworm pupaprotein medium remains yellow and clear after high pressure steam sterilization.The bacterial culture and identification experiments show that silkworm pupaprotein can completely instead of peptone as nitrogen source and carbon source for the growth of bacteria.The silkworm pupaprotein medium can ensure the rapid accumulation of biomass while maintaining the normal growth and morphology of bacteria.Silkworm pupa protein has many advantages such as source abundance,moderate price,and industrialized processing,and it can be used as a new cheap substitute instead of peptone to develop new bacterial medium.

关 键 词：蚕蛹蛋白 蛋白胨 新型细菌培养基 细菌培养 

分 类 号：Q812[生物学—生物工程]