磷酸化p38介导低切应力诱导的血管内皮细胞Bmi-1表达  

Phosphorylated p38 mediates the expression of Bmi-1 in vascular endothelial cells induced by low shear stress

作　　者：兰祥星 王汉琴 操传斌 张振建 

机构地区：[1]湖北医药学院附属随州医院心血管内科,湖北省随州市441300 [2]湖北医药学院附属随州医院转化医学研究中心,湖北省随州市441300

出　　处：《中国动脉硬化杂志》2018年第2期122-126,共5页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金项目(31670961);湖北省科技厅自然科学基金项目(2015CFB186)

摘　　要：目的 探讨低切应力（LSS）对人脐静脉内皮细胞（HUVEC）中Bmi-1表达的影响及其可能的机制。方法原代培养HUVEC,免疫荧光检测Bmi-1在细胞中的定位;运用平行平板流动腔系统,给HUVEC加载0.5 Pa低切应力0.5 h、1 h、2 h和4 h,以无切应力加载为对照组,用实时定量RT-PCR检测Bmi-1 mRNA表达,用Western blot检测Bmi-1蛋白表达,利用p38特异性抑制剂SB2219探讨信号转导途径。结果 免疫荧光观察发现Bmi-1主要分布在HUVEC胞核中;HUVEC在LSS作用0.5 h后Bmi-1表达即明显增强,随着作用时间延长（1 h、2 h、4 h）,Bmi-1mRNA及蛋白表达逐渐降低;切应力能显著激活磷酸化p38表达;SB2219可以明显抑制Bmi-1表达。结论 LSS可诱导HUVEC中Bmi-1表达,其表达量与刺激时间长短密切相关,这种作用可能通过p38信号调节。Ahn To investigate the effect of low shear stress （LSS） on the expression of Bmi-1 in human umbili- cal vein endothelial ceils （HUVEC） and its possible mechanism. Methods Human umbilical vein endothelial cells were cultured in vitro. Immunofluorescence was used to detect the localization of Bmi-1 in the cells. 0.5 h, 1 h, 2 h and 4 h were loaded into human umbilical vein endothelial cells by parallel plate flow chamber system. The expression of Bmi- 1 mRNA was detected by real-time quantitative RT-PCR. The expression of Bmi-1 protein was detected by Western blot. Specific signal inhibitor SB2219 was used to investigate the signal transduction pathway. Results Immunofluorescence observation showed that Bmi-1 was mainly distributed in the nucleus of human umbilical vein endothelial cells. The expression of Bmi-1 was significantly increased after 0.5 h of LSS, the expression of Bmi-1 mRNA and protein decreased grad- ually with the prolongation of the time （ 1 h, 2 h and 4 h） ; the shear stress could significantly activate phosphorylated p38 expression; SB2219 could significantly inhibit the expression of Bmi-1. Conclusion LSS can induce the expression of Bmi- 1 in human umbilical vein endothelial cells, the expression of Bmi- 1 is closely related to the length of stimulation, this effect may be regulated by p38 signal.

关 键 词：血管内皮细胞 BMI-1 低切应力 p38信号转导 

分 类 号：R363[医药卫生—病理学]