分拣受体Sortilin促进巨噬细胞内脂质蓄积  

Sortilin accelerates the lipid accumulation in THP-1 macrophage

作　　者：钟丽园 彭田红[1] 高安博 万炜 陈熙[1] 王佐[2] 唐志晗[2] 王莉[1] 欧阳宇甲 吕运成[1] 

机构地区：[1]南华大学医学院应用解剖与生殖医学研究所,湖南省衡阳市421001 [2]南华大学医学院心血管疾病研究所,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2018年第2期139-143,共5页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金项目(81770460);加拿大萨斯喀彻温省卫生研究基金会博士后基金项目(SHRF4144);湖南省教育厅资助科研项目(10C1178);南华大学博士启动资金项目(2014XQD37);2015年度“大学生研究性学习和创新性实验计划”校级项目

摘　　要：目的 探讨分拣受体Sortilin对THP-1巨噬细胞内脂质代谢的影响。方法 用氧化型低密度脂蛋白（ox-LDL）孵育THP-1巨噬细胞。Western blot检测荷脂后THP-1巨噬细胞中Sortilin表达变化;THP-1巨噬细胞Sortilin高表达和沉默后,液体闪烁计数仪检测胞内胆固醇流出,高效液相色谱法检测细胞内脂质含量,油红O染色观察胞内脂滴情况。结果 THP-1巨噬细胞Sortilin表达呈ox-LDL剂量和时间依赖性增加;以50 mg/L ox-LDL处理24 h后THP-1巨噬细胞Sortilin表达水平达到高峰。与对照组相比,Sortilin过表达后巨噬细胞胆固醇流出减少,胞内脂质含量增加且脂滴明显增多,而Sortilin沉默后则刚好出现相反的结果。结论 Sortilin抑制巨噬细胞内胆固醇流出,促进胞内脂质蓄积。Aim To explore the effect of sorting receptor Sortilin on lipid metabolism in THP-1 macrophages. Methods THP-1 macrophages were incubated with oxidized low density lipoprotein （ox-LDL）. Change of Sortilin ex- pression in lipid-loaded THP-1 maerophages were detected by Western blot. Under the overexpression and silence of Sorti- lin in THP-1 macrophages, intracellular cholesterol efflux was measured by liquid scintillation counting apparatus, intracel- lular lipid content was detected by high performance liquid chromatography, and the situation of intracellular lipid droplets was observed by oil red O staining. Results The expression of Sortilin in THP-1 macrophages was increased in the manners of dose- and time-dependence of ox-LDL. The expression level of Sortilin in THP-1 macrophages reached the peak after 24 hours treatment with 50 mg/L ox-LDL. Compared with the control group, after Sortilin overexpression, the cholesterol efflux of maerophages was decreased, the intraeellular lipid content was increased, and the lipid droplets was in- creased significantly, while Sortilin was silent, it just happened to be the opposite. Conclusion Sortilin inhibits the cholesterol efflux of macrophages and promotes the accumulation of intracellular lipid.

关 键 词：SORTILIN 巨噬细胞 脂质蓄积 氧化型低密度脂蛋白 动脉粥样硬化 

分 类 号：R363[医药卫生—病理学]