β-catenin和IQGAP1的调控环路及其在肠癌细胞增殖中的作用  

A β-catenin/IQGAP1 regulatory feedback loop and its effects on the proliferation of colon cancer cells

作　　者：徐焕基 夏洪伟[2] 唐秋琳[2] 毕锋[1,2] 

机构地区：[1]四川大学华西医院肿瘤中心腹部肿瘤科,成都610041 [2]四川大学华西医院肿瘤分子靶向治疗实验室,成都610041

出　　处：《生物医学工程学杂志》2018年第1期81-86,共6页Journal of Biomedical Engineering

基　　金：国家自然科学基金(81572731)

摘　　要：本文主要研究β连环蛋白(β-catenin)和含IQ基序的GTP激酶活化蛋白1(IQGAP1)的相互调控,及其在肠癌细胞增殖中的功能。利用细胞转染技术改变肠癌细胞SW1116中的β-catenin或者IQGAP1表达后,通过蛋白质印迹法分别检测SW1116细胞中IQGAP1和β-catenin的表达情况。利用CCK-8细胞增殖实验检测干扰下调IQGAP1对β-catenin促进SW1116细胞增殖的影响。结果发现在SW1116细胞中干扰β-catenin可以下调IQGAP1表达,过表达β-catenin可以上调IQGAP1并加强有丝分裂原激活蛋白激酶(MAPK)通路;干扰IQGAP1在SW1116细胞中反而引起β-catenin上升;同时CCK-8细胞增殖实验发现干扰IQGAP1减弱了β-catenin的促肠癌细胞增殖作用。本研究表明β-catenin与IQGAP1形成负向反馈调控环路,在调控肠癌细胞的增殖中发挥着重要功能。The aim of this article is to study the regulatory feedback loop between β-catenin and IQ motif containing GTPase activating protein 1 （IQGAP1）, as well as the effect of this regulation loop in colon cancer cell proliferation. Western blot was used to detect the expression of IQGAP 1 and β-catenin after changing their expression respectively by transfection in SW1116 cells. CCK-8 cell proliferation assay was used to detect the effect of IQGAP1 involved in the proliferation of SWll 16 cells promoted by β-catenin. The results of Western blot indicated that β-catenin could positively regulate IQGAP1, while IQGAP1 silencing could up-regulate β-catenin, forming a negative feedback loop. The results of CCK-8 showed that IQGAP1 silencing inhibited β-catenin-mediated proliferation in SW1116 cells. In conclusion, our research reveals a negative regulatory feedback loop between β-catenin and IQGAP1 which has a remarkable effect on the proliferation ability of colon cancer cells.

关 键 词：Β连环蛋白 含IQ基序的GTP激酶活化蛋白1 有丝分裂原激活蛋白激酶通路 细胞增殖 

分 类 号：R735.3[医药卫生—肿瘤]