鸦胆子油注射液对ⅠB期子宫颈癌术后放疗患者抗肿瘤免疫应答功能的影响  

Influence of Fructus bruccae emulsion on the anti-tumor immunity in ⅠB stage cervical carcinoma patients undergoing postoperative radiotherapy

作　　者：张杰[1] 朱彩丹 叶朝辉[1] 

机构地区：[1]宁波市妇女儿童医院药剂科,浙江宁波315012 [2]宁波市妇女儿童医院妇产科,浙江宁波315012

出　　处：《实用肿瘤杂志》2018年第1期91-94,共4页Journal of Practical Oncology

摘　　要：目的探讨鸦胆子油注射液治疗对ⅠB期子宫颈癌术后放疗患者抗肿瘤免疫应答功能的影响。方法收集接受手术治疗的ⅠB期子宫颈癌患者80例,按照单盲随机对照法将其分为接受术后单纯放疗的对照组40例、接受术后鸦胆子油注射液联合放疗的观察组40例,持续治疗30 d。治疗前、治疗30 d后,采用流式细胞仪测定两组患者外周血中T淋巴细胞免疫,B细胞、自然杀伤(natural killer,NK)细胞、巨噬细胞免疫等指标水平,采用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)测定外周血中Th1/Th2免疫指标含量。结果治疗前,两组外周血中T淋巴细胞免疫比较,B细胞、NK细胞和巨噬细胞免疫比较,Th1/Th2免疫水平比较,差异均无统计学意义(均P<0.05)。治疗30 d后,观察组外周血中CD4^+和CD4^+/CD8^+水平均高于对照组,CD8^+水平低于对照组,CD16^+CD56^+、CD20^+和CD68^+水平均高于对照组(均P<0.05);观察组外周血中干扰素-γ(interferon-γ,IFN-γ)和白介素-2(interleukin-2,IL-2)含量均高于对照组,IL-4和IL-10含量均低于对照组(均P<0.05)。结论鸦胆子油注射液治疗可增强子宫颈癌术后放疗患者的抗肿瘤免疫应答功能。Objective To study the influence of Fructus bruccae emulsion on the anti-tumor immunity in ⅠB stage cervical carcinoma patients undergoing postoperative radiotherapy. Methods Eighty cases of ⅠB stage cervical carcinoma patients who have received operation were enrolled and assigned to two groups according to the single blind randomized control method,with 40 patients in each group. The control group received postoperative radiotherapy only,and the observation group received postoperative Fructus bruccae emulsion combined with radiotherapy. Both groups were treated for 30 d.Both before and 30 d after treatment,peripheral blood T lymphocyte immunity and B cell/natural killer（ NK） cell/macrophage immunity were measured by flow cytometry,and peripheral blood Th1/Th2 immunity was detected by enzyme linked immunosorbent assay（ ELISA）. Results Before the treatment,there were no significant differences in peripheral blood T lymphocyte immunity,B cell/NK cell/macrophage immunity,and Th1/Th2 immunity between the two groups（ all P〉0. 05）. However,30 d after treatment,peripheral blood CD4^＋and CD4^＋/CD8^＋levels were higher in the observation group than those in the control group（ both P〈0. 05）. The level of CD8^＋level was decreased,yet the levels of CD16^＋CD56^＋,CD20^＋and CD68^＋were increased in the observation group,compared with the control group（ all P〈0. 05）. Moreover,peripheral blood interferon-γ（ IFN-γ） and interleukin-2（ IL-2） levels were higher,whereas IL-4 and IL-10 levels were lower in the observation group（ all P〈0. 05）. Conclusion Fructus bruccae emulsion can enhance the anti-tumor immune response function in cervical carcinoma patients undergoing postoperative radiotherapy.

关 键 词：宫颈肿瘤/外科学 宫颈肿瘤/放射疗法 宫颈肿瘤/药物疗法 鸦胆子/治疗应用 免疫疗法 

分 类 号：R737.33[医药卫生—肿瘤]