PTEN及BCL-2在急性髓系白血病中的表达及意义  

Expression and Significance of PTEN and BCL-2 in Acute Myeloid Leukemia

作　　者：孙巨勇[1] 牟娜[1] 牟佳 李伟[1] 张长庚[1] 王冬梅[3] 

机构地区：[1]衡水市哈励逊国际和平医院检验科,河北衡水053000 [2]衡水市妇幼保健院检验科 [3]衡水市哈励逊国际和平医院血液科,河北衡水053000

出　　处：《中国实验血液学杂志》2018年第1期121-125,共5页Journal of Experimental Hematology

摘　　要：目的:探讨人第10号染色体缺失的磷酸酶及张力蛋白同源蛋白的基因(PTEN)及B细胞淋巴瘤/白血病-2(BCL-2)在急性髓系白血病(AML)中的表达及意义。方法:采用Real-time PCR和Western blot法检测80例AML(包括56例初治患者,16例缓解患者,8例复发患者)及30例非血液病患者骨髓中PTEN及BCL-2的mRNA及蛋白表达水平,并分析二者与临床病理参数的关系。结果:初治和复发组患者骨髓细胞PTEN mRNA及蛋白表达水平均显著低于对照组及缓解组(P<0.01),BCL-2 mRNA及蛋白表达量均显著高于对照组及缓解组(P<0.01)。PTEN与BCL-2 mRNA表达水平与AML患者年龄,性别及白细胞计数无相关性(P>0.05)。AML患者中PTEN与BCL-2蛋白及mRNA表达水平均呈负相关(r=-0.432,r=-0.569)。结论:PTEN及BCL-2参与AML的发生发展,并对AML的化疗效果起着指示作用。Objective： To explore the expression of phosphatase and tensin homolog deleted on chromosome ten（ PTEN） and B-cell lymphoma/leukemia-2（ BCL-2） in acute myeloid leukemia（ AML） and its significance. Methods：The expression levels of PTEN and BCL-2 mRNA and protein in bone marrowsamples from 80 AML patients including56 de novo patients,16 patients in remission,8 relapsed patients and 30 cases of non-hematologic diseases（ as control）were detected by real-time PCR and Western blot, respectively, and the relationship between PTEN and BCL-2 expression and clinical pathological parameter was analyzed. Results： The expression levels of both mRNA and protein of PTEN in newly diagnosed AML group and relapse group were significantly lower than those in the control and remission group（ P〈0. 01）. The expression levels of both mRNA and protein of BCL-2 in newly diagnosed group and relapse group were significantly higher than those in the control and remission group（ P〈0. 01）. The mRNA expression of PTEN and BCL-2 was did not related with the age,sex and white blood count in AML patients. The expression levels of PTEN negatively correlated with expression BCL-2 with AML（ r =-0. 432,r =-0. 569）. Conclusion： PTEN and BCL-2 participate in the occurrence and development of AML,and may be used as indicators for the evaluation of chemotheraeutic efficacy.

关 键 词：急性髓细胞白血病 人第10号染色体缺失的磷酸酶及张力蛋白同源蛋白的基因(PTEN) B细胞淋巴瘤/白血病-2(BCL-2) 

分 类 号：R733.71[医药卫生—肿瘤]