ADAM17基因RNAi重组慢病毒对小鼠内毒素血症的抑制作用  

Inhibitory Effect of the ADAM17 shRNA Lentivirus Vector to Endotoxemia in Mice

作　　者：何兵[1] 庹虎[1] 连文静[1] 李小鸥[1] 

机构地区：[1]武汉大学人民医院儿科

出　　处：《武汉大学学报（医学版）》2018年第2期219-222,共4页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:81000094);湖北省自然科学基金资助项目(编号:2012FB04418)

摘　　要：目的：建立小鼠内毒素血症模型,观察ADAM17shRNA的重组慢病毒对炎症的抑制作用,为人工干预炎症过程提供依据和手段。方法：将ADAM17shRNA的重组慢病毒作用于小鼠内毒素血症模型,通过流式细胞术检测小鼠腹腔巨噬细胞mTNF-α的表达,并取肝、肺、肾组织行HE染色,观察重组慢病毒对炎症的抑制作用。结果：动物实验证明重组慢病毒组小鼠腹腔巨噬细胞膜表面mTNF-α增加,常规病理切片HE染色显示该慢病毒可降低LPS诱发的小鼠肝、肾、肺组织的炎症反应。结论：ADAM17重组慢病毒降低了sTNF-α的分泌,对炎症有明显的抑制作用,为抗炎药物的设计和改造提供了新的依据和方法。Objective： To study the effect of ADAM17 shRNA lentivirus vector on TNF-a secretion and develop an approach to interfere inflammation processes. Methods： The Kunming mice were con- dueted with the endotoxic shock models, then were divided into ADAM17 shRNA group and PBS control group. The mTNF-α of the macrphage cells was detected by FCM and the histopathology HE section was performed to study the inflammatory changes of the liver, kidndy and lung. Results： The mice exposed to the ADAM17 shRNA lentivirus prior to inducing endotoxemia with LPS had fewer signs of inflammation and less tissue damage than control mice. Conclusion. The ADAM17 shRNA lentivirus is an effective antagonist of TACE and inhibits the sTNF-α release, which indicated ADAM17 is an novel target for inflammation theraphy.

关 键 词：RNA干扰 ADAM17 肿瘤坏死因子Α 炎症 

分 类 号：R364.5[医药卫生—病理学]