一株寄生曲霉SF-2对甘蔗绵蚜的感染作用及其生物学特性初步研究  

The Infection of a SF-2 strain of Aspergillus sp. on Sugarcane Aphid and Preliminary Study on Its Biological Characteristics

作　　者：代园凤 张承 喻会平[1] 梁银 郑登峰 吴小毛 龙友华 王秋萍[3] 张丽娟 

机构地区：[1]贵州省烟草公司毕节市公司,贵州毕节551700 [2]贵州农业职业学院,贵州贵阳551400 [3]贵州大学农学院,贵州贵阳550025

出　　处：《云南农业大学学报（自然科学版）》2017年第6期1006-1011,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：贵州省优秀青年科技人才基金[黔科合平台人才(2017)5616];贵州省科技厅农业攻关项目[黔科合NY字(2011)3024];中国烟草总公司贵州省公司科技项目(201713;201509;201414)

摘　　要：【目的】为利用寄生曲霉SF-2控制甘蔗绵蚜提供理论依据。【方法】采用孢子悬浮液喷雾法测定了寄生曲霉SF-2对甘蔗绵蚜的感染作用,并利用菌丝生长速率法和孢子萌发法对其生物学特性进行了初步研究。【结果】寄生曲霉SF-2可感染6种同翅目害虫,且对甘蔗绵蚜、吹绵蚧和矢间蚧具有良好的感染作用,喷雾孢子悬浮液7 d后校正感染率分别达98.33%、98.25%和96.67%。寄生曲霉SF-2生长最适温度为32℃左右、pH为4,在周期性光暗交替中生长良好;且其孢子最适萌发温度为32℃左右,紫外照射能显著影响孢子萌发。【结论】寄生曲霉在防控农作物害虫上潜力较大。[ Purpose ] Our aim was to provide the oretical basis for control of sugarcane aphid by u- sing Aspergillus sp. SF-2. [ Methods ] The infection ratio of Aspergillus sp. SF-2 to Ceratovacuna lan- igera （Zehntner） was tested by spore suspension spray method and its biological characteristics were preliminary studied by myeelium growth rate method and spore germination method. [ Results] Asper- gillus sp. SF-2 had infection effects on six kinds of homopteran pests, the infection effects were better on C. lanigera, Icerya purchasi Maskell and Unaspis yanonensis （Kuwana） , the adjusted infection rate were 98.33%, 98.25% and 96.67% spraying spore suspension 7 days after, respectively. At 32 ℃, pH 4.0 and light dark alternation, the mycelial growth of Aspergillus sp. SF-2 was the fastest. The besttemperature of germination was about 32 ℃, and UV irradiation could significantly affect the germina- tion of spores. [ Conclusion] Aspergillus sp. has great potential in control of crop pests.

关 键 词：寄生曲霉 甘蔗绵蚜 感染 生物学特性 

分 类 号：S435.66[农业科学—农业昆虫与害虫防治]