CDX2、NF-κB和MUC2在Barrett食管中的表达及意义  

Expressions and significance of CDX2,NF-κB and MUC2 in Barrett esophagus

作　　者：蒋丽[1] 张桂英[2] 

机构地区：[1]湖南省长沙市第四医院消化内科,长沙市410006 [2]中南大学湘雅医院消化内科,长沙市410008

出　　处：《微创医学》2018年第1期17-19,41,共4页Journal of Minimally Invasive Medicine

摘　　要：目的研究尾型同源框转录因子-2(CDX2)、核因子-κB(NF-κB)和粘蛋白-2(MUC2)在Barrett食管(BE)中的表达及意义。方法采用随机对照法比较普通白光内镜及NBI模式下活检BE并肠化(IM)的检出率。选取BE(经NBI筛选)、正常食管黏膜及食管腺癌(EAC)采用免疫组化方法检测CDX2、NF-κB和MUC2的表达。结果 NBI组BE并IM的检出率为65.8%(25/38),显著高于普通内镜的34.2%(13/38),差异有统计学意义(P<0.05)。正常黏膜组、BE组和EAC组CDX2阳性率分别为0、73.3%(22/30)、33.3%(10/30);NF-κB阳性率依次为6.7%(2/30)、66.7%(20/30)、40.0%(12/30);MUC2阳性率依次为0、60.0%(18/30)、26.7%(8/30)。三组CDX2、NF-κB、MUC2的阳性率比较,差异均有统计学意义(P<0.05)。CDX2阳性率与NF-κB、MUC2阳性率呈正相关,r值分别为0.869、0.820。结论 NBI能有效提高BE并IM的病理检出率。CDX2、NF-κB、MUC2的激活可能导致BE肠上皮化生及后续恶变。ObjectiveTo investigate expressions and significance of caudal type homeo box transcription factor 2 （CDX2）, nuclear factor kappa B （NF-κB） and mucin 2 （MUC2） in Barrett esophagus（BE） . MethodsThe detection rate of BE complicated with intestinal metaplasia （IM） was compared between biopsy with conventional white light endoscopy and biopsy with narrow band imaging（NBI） mode on the basis of the randomized controlled method. The expressions of CDX2, NF-κB and MUC2 were detected in BE （after screening with NBI） , normal esophageal mucosa and esophageal adenocarcinoma（EAC） tissues by immunohistochemical method. ResultsThe detection rate of BE complicated with IM in the NBI group was higher than that in the endoscopy group[65.8%（25/38） vs 34.2%（13/38）, P〈0.05]. The positive rates of CDX2 in the normal esophageal mucosa, BE and EAC tissues were 0, 73.3%（22/30）and 33.3%（10/30） respectively. The positive rates of NF-κB in the normal esophageal mucosa, BE and EAC tissues were 6.7%（2/30）, 66.7%（20/30）and 40.0%（12/30）respectively. The positive rates of MUC2 in the normal esophageal mucosa, BE and EAC tissues were 0, 60.0%（18/30）and 26.7%（8/30） respectively. There were significant differences in the positive rates of CDX2, NF-κB and MUC2 among the three tissues（P〈0.05）. The positive rates of CDX2 positively correlated to the positive rate of NF-κB（r=0.869）or MUC2（r=0.820）. ConclusionNBI can effectively improve the pathological detection rate of BE complicated with IM. Activation of CDX2, NF-κB and MUC2 may induce IM and malignant transformation in BE.

关 键 词：BARRETT食管 尾型同源框转录因子-2 核因子-ΚB 粘蛋白-2 窄带成像内镜 

分 类 号：R571[医药卫生—消化系统]