金丝草化学成分及其体外抗HBV活性  

Chemical constituents from Pogonatherum crinitum and their in vitro anti-HBV activities

作　　者：袁晓旭[1] 李洪波[1] 尹志峰[1] 王慧娜 赵桂琴[1] 

机构地区：[1]承德医学院中药研究所,河北省中药研究与开发重点实验室,河北承德067000

出　　处：《中成药》2018年第2期363-368,共6页Chinese Traditional Patent Medicine

基　　金：河北省教育厅重点项目(ZD2016061); 河北省中医药管理局项目(2015034); 河北省高校重点学科建设项目(冀教高[2013]4)

摘　　要：目的研究金丝草的化学成分及其体外抗HBV活性。方法金丝草70%乙醇提取物的乙酸乙酯和正丁醇部位采用AB-8大孔树脂、硅胶、Sephadex LH-20进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。HepG2.2.15细胞模型法测其体外抗HBV活性。结果从中分离得到8个化合物,分别鉴定为6,8,4'-三羟基-7,3'-二甲氧基异黄酮(1)、槲皮素-7,4'-二甲醚-5-O-β-D-吡喃葡萄糖苷(2)、8-[1-(3,4-二羟基苯基)乙基]槲皮素(3)、金圣草素-7-O-α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃葡萄糖苷(4)、山柰酚-3-O-(2″,3″-二-O-E-p-香豆酰基)-α-L-吡喃鼠李糖苷(5)、1,3,7-三羟基呫吨酮-2-C-β-D-吡喃葡萄糖苷(6)、木犀草素-6-C-β-D-吡喃波依文糖苷(7)、木犀草素-6-C-β-D-吡喃葡萄糖苷(8)。化合物3、5、7、8对细胞分泌表面抗原(HBsAg)有一定的抑制作用,IC50分别为96.6、127.1、72.4、54.5μg/m L;化合物7、8对细胞分泌e抗原(HBe Ag)有一定的抑制作用,IC50分别为81.0、61.3μg/m L。结论化合物1~6为首次从金发草属植物中分离得到,化合物3、5、7、8有一定的体外抗HBV活性。AIM To study the chemical constituents from Pogonatherum crinitum（ Thunb.） Kunthand and their in vitro anti-HBV activities. METHODS The ethyl acetate and n-butanol fractions of 70% ethanol extract from P. crinitum were isolated and purified by AB-8 macroporous resin,silica column and Sephadex LH-20,then the structures of obtained compounds were identified by physicochemical properties and spectral data. The in vitro anti-HBV activities of the isolated compounds were tested in the model of HepG2. 2. 15 cells. RESULTS Eight compounds were isolated and identified as 6,8,4＇-trihydroxy-7,3＇-dimethoxyisoflavone（ 1）,quercetin-7,4＇-dimethylester-5-O-β-D-glucopyranoside（ 2）,8-（ 1-（ 3,4-dihydroxyphenyl） ethyl） quercetin（ 3）,chrysoeiml-7-O-α-L-rhamnopyranosyl-（ 1 → 2）-β-D-glucopyranoside（ 4）, kaempferol-3-O-（ 2″, 3″-di-O-E-p-coumaroyl）-α-L-rhamnopyranoside（ 5）,1,3,7-trihydroxyxanthone-2-C-β-D-glucopyra-noside（ 6）,luteolin-6-C-β-D-boivinopyranoside（ 7）,luteolin-6-C-β-D-glucopyranoside（ 8）. Compounds 3,5,7,8 had certain inhibitory effects on HBs Ag with the IC50 values of 96. 6,127. 1,72. 4 and 54. 5 μg/mL. Compounds 7,8 had certain inhibitory effects on HBe Ag with the IC50 values of 81. 0,61. 3 μg/m L. CONCLUSION Compounds 1-6 are isolated from genus Pogonatherum for the first time,compounds 3、5、7、8 have certain in vitro anti-HBV activities.

关 键 词：金丝草 化学成分 分离鉴定 体外抗HBV活性 

分 类 号：B284.1[哲学宗教—中国哲学]