丙氨酰-谷氨酰胺缓解Diquat诱导的断奶仔猪氧化损伤的影响  

Effects of Alanyl-Glutamine Alleviate Oxidative Damage Induced by Diquat of Weaned Piglets

作　　者：辛向荣[1] 叶亚玲[1] 游金明[1] 贺琴[1] 邓宸玺 

机构地区：[1]江西农业大学,江西省动物营养重点实验室,江西省营养饲料开发工程中心,南昌330045

出　　处：《动物营养学报》2018年第2期658-666,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31360554);江西省科技落地计划项目(KJLDl4027)

摘　　要：本研究旨在探讨丙氨酰-谷氨酰胺(Ala-Gln)缓解Diquat诱导的断奶仔猪氧化损伤的影响。试验采用双因子设计,选取24头健康状况良好、胎次相近的21日龄断奶仔猪,首先随机分成2个组,每组12个重复,每个重复1头猪,2个组分别饲喂基础饲粮和基础饲粮+0.3%Ala-Gln的试验饲粮。预饲喂7 d后,在前期饲喂基础上,将仔猪分成4个组,每组6个重复,每个重复1头猪,分别为基础饲粮组、基础饲粮+0.3%Ala-Gln组、基础饲粮应激组、基础饲粮+0.3%Ala-Gln应激组。应激组通过腹腔注射8 mg/kg BW Diquat模拟仔猪氧化应激,而未应激组则注射等量的灭菌生理盐水。试验期7 d。结果表明:1)仔猪氧化应激状态下,与基础饲粮应激组相比,饲粮中添加Ala-Gln显著提高了血清谷氨酰胺(Gln)、谷胱甘肽(GSH)含量和谷胱甘肽过氧化物酶(GSH-Px)、总超氧化物歧化酶(T-SOD)活性以及总抗氧化能力(T-AOC)(P<0.05)。2)仔猪正常生理状态和氧化应激状态下,与相应基础饲粮组和基础饲粮应激组相比,饲粮中添加Ala-Gln显著提高了空肠GSH-Px活性和T-AOC(P<0.05),显著降低了丙二醛(MDA)含量(P<0.05);饲粮中添加Ala-Gln显著提高了肝脏GSH-Px活性和T-AOC(P<0.05),显著降低了MDA含量(P<0.05)。3)仔猪氧化应激状态下,与基础饲粮应激组相比,饲粮中添加Ala-Gln显著提高了肝脏中谷胱甘肽过氧化物酶4(GPx4)mRNA表达量(P<0.05),显著降低了超氧化物歧化酶1(SOD1)mRNA表达量(P<0.05)。由此可知,仔猪正常生理状态和氧化应激状态下,饲粮中添加Ala-Gln可提高仔猪抗氧化能力,降低MDA含量,从而减缓氧化应激对断奶仔猪机体组织的损伤;且在氧化应激状态下效果更为显著。This experiment was conducted to study the effects of alanyl-glutamine （Ala-Gln） alleviate oxida- tive damage induced by Diquat of weaned piglets. The method of double-factor design is adopted. Twenty four piglets with health condition and similar parity weaned at 21 days of age were randomly divided into 2 groups with 12 replicates in each group and 1 pig in each replicate. Pigs in two groups were fed control diet and control diet＋0.30% Ala-Gln, respectively. After 7 days pre feeding, based on the prophase feeding, the 24 piglets were randomly divided into 4 groups with 6 replicates in each group and 1 pig in each replicate. The four groups were basal diet group, basal diet＋0.3% Ala-Gln group, basal diet stress group and basal diet＋0.3% Ala-Gln stress group, respectively. Piglet oxidative stress was simulated by intraperitoneal injection of 8 mg/kg BW Diquat, and the unstressed groups were injected with the same volume of sterilized saline. The experiment lasted for 7 days. The results showed as follows ： 1 ） piglets under oxidative stress state, compare with the basal diet stress group, dietary supplemented with Ala-Gln significantly increased the contents of glutamine （Gin）, glutathione （GSH） and activities of glutathione peroxidase （GSH-Px）, total superoxide dismutase （T-SOD） and total antioxidant capacity （T-AOC） in serum （P〈0.05）. 2） Piglets under normal physiological state and oxidative stress state, compared with corresponding basal diet group and basal diet stress group, dietary supple- mented with Ala-Gln significantly increased the GSH-Px activity and T-AOC in jejunum （P〈0.05）, and sig- nificantly decreased the malondialdehyde （MDA） content （P〈0.05） ; dietary supplemented with Ala-Gln sig- nificantly increased the GSH-Px activity and T-AOC in liver （P〈0.05）, and significantly decreased the malon- dialdehyde （MDA） content （P〈0.05）. 3） Piglets under oxidative stress state, compare with the basal diet stress group, dietary supplem

关 键 词：氧化应激 Ala-Gln 仔猪 抗氧化能力 

分 类 号：S828[农业科学—畜牧学]