T细胞抗原受体（TCR）标志物βF1及TCRγ在T淋巴母细胞淋巴瘤/白血病中的表达  

Expression of βF1 and T cell receptor γ in T lymphoblastic lymphoma/leukemia

作　　者：张芬[1] 罗东兰[1] 陈玉[1] 武鸿美[1] 严金海 骆新兰[1] 何娇[1] 罗陆桥 刘艳辉[1] 

机构地区：[1]广东省人民医院(广东省医学科学院)病理医学部病理科,广州510080

出　　处：《中华病理学杂志》2018年第2期119-122,共4页Chinese Journal of Pathology

基　　金：国家临床重点专科建设项目;广东省医学科学技术研究基金（B2017022）

摘　　要：摘要目的检测T细胞抗原受体（TCR）标志物βF1及TCRγ在T淋巴母细胞淋巴瘤/白血病（T－LBL/ALL）中的表达情况，并探讨其临床病理特征。方法收集2010至2016年广东省人民医院病理科51例确诊为T－LBL/ALL的病例，采用免疫组织化学EnVision法，检测βF1及TCRγ在T－LBL/ALL表达情况并进行统计学分析。结果51例T－LBL/ALL中儿童及青少年患者13例，成人患者38例。βF1及TCRγ在T－LBL/ALL的表达率分别为27.5%（14/51）及15.7%（8/51）。成人患者（≥18岁）在αβ T－LBL/ALL、TCR沉默型T－LBL/ALL及γδ T－LBL/ALL占比分别为7/14、79.3%（23/29）及8/8，其差异具有统计学意义（P＝0.023）。而性别、乳酸脱氢酶值、骨髓侵犯及临床分期在3组中差异均无统计学意义（P〉0.05）。8例γδ T－LBL/ALL中6例为CD4^－/CD8^－表型，14例αβ T－LBL/ALL中7例为CD4^＋/CD8^＋表型。CD4/CD8表达情况在3组中差异有统计学意义（P〈0.01）。结论γδ T－LBL/ALL仅发生于成人患者，以CD4^－/CD8^－表型为主；αβ T－LBL/ALL各年龄阶段均可发生，儿童及青少年患者发生率更高，以CD4^＋/CD8^＋表型最多见。ObjectiveTo evaluate the expression of βF1 and T cell receptor （TCR）γ in T lymphoblastic lymphoma/leukemia（T-LBL/ALL）, and investigate the clinicopathological features.MethodsFifty-one cases of T-LBL/ALL were collected at Guangdong General Hospital from 2010 to 2016, the expression of βF1 and TCRγ was assessed by immunohistochemistry.ResultsThere were 13 cases of children and adolescents, and 38 cases of adults. The expression rates of βF1 and TCRγ were 27.5%（14/51） and 15.7%（8/51） respectively. The proportion of adults in αβ T-LBL/ALL, TCR-silent T-LBL/ALL and γδ T-LBL/ALL was 7/14, 79.3%（23/29）and 8/8 respectively, and the difference was significant （P=0.023）. There was no statistical difference in sex, LDH, bone marrow involvement and Ann arbor stage among these three groups（P〉0.05）. γδ T-LBL/ALLs included 6 cases of CD4^-/CD8^- phenotype, whereas αβ T-LBL/ALL included 7 cases of CD4^＋ /CD8^＋ phenotype. There was significant difference in CD4/CD8 expression among these three groups（P〈0.01）.Conclusionsγδ T-LBL/ALL occurred only in adults, with predominantly CD4^-/CD8^- phenotype. αβ T-LBL/ALL occurred more common in children and adolescents, with predominantly CD4^＋ /CD8^＋ phenotype.

关 键 词：前体细胞淋巴母细胞白血病淋巴瘤 基因 T细胞抗原受体 免疫组织化学 

分 类 号：R733[医药卫生—肿瘤]