一步酶裂解法生产7-氨基头孢烷酸的工艺改进  

Improvement in one step enzymatic process of 7-aminocephalosporanic acid

作　　者：梅玉龙 吴成军 马阳[1] 解凤 王仕科 孙铁民[1] 

机构地区：[1]沈阳药科大学制药工程学院,辽宁沈阳110016 [2]石药集团中诺药业(石家庄)有限公司,河北石家庄050051

出　　处：《中国药物化学杂志》2018年第1期45-47,共3页Chinese Journal of Medicinal Chemistry

摘　　要：目的研究一步酶裂解法生产7-氨基头孢烷酸(7-ACA)的合成工艺。方法依据生物催化7-ACA法的反应机制,在7-ACA一步酶法裂解反应中,分别以氨水和碳酸钠作为反应体系的p H值调节剂,对所得的实验数据进行比较分析。结果与结论以碳酸钠代替氨水作p H值调节剂,反应后7-ACA浓度、反应物残留浓度、杂质及收率等指标均无差别,但是用碳酸钠代替氨水裂解时氨氮值平均降低了80.33%,该方法绿色环保,对环境污染小,适合工业化生产。Based on the reaction mechanism of bio-catalytic process of 7-aminocephalosporanic acid, specifically in the one-step enzymatic conversion of cephalosporin C to 7-aminocephalosporanic acid, sodium carbonate was used in place of ammonia as a pH adjuster of the reaction. By comparing the experimental data of sodium carbonate and ammonia, the results showed that sodium carbonate can replace ammonia to adjust the pH value of the 7-aminocephalosporanic acid reaction, and the quality index of the reaction product is not obviously different, while the reaction system ammonia nitrogen values decreased significantly. This reduced the pressure on environmental protection.

关 键 词：7-氨基头孢烷酸(7-ACA) 一步酶法 裂解 机制 

分 类 号：R914[医药卫生—药物化学]