人血浆Nav1.5通道蛋白ELISA检测方法的建立  

Establishment of ELISA method for human plasma Nav1.5 channel protein detection

作　　者：陈瑞庆[1] 王锃[1] 蓝瑞隆[1] 陈锦容 陈纬[1] 张鲁榕[1] 

机构地区：[1]福建医科大学附属第一医院中心实验室,福州350005

出　　处：《福建医药杂志》2018年第1期108-110,共3页Fujian Medical Journal

基　　金：福建省教育厅中青年教师教育科学项目(JA15203)

摘　　要：目的建立一种高效特异、灵敏度高的ELISA方法检测人血浆Nav1.5(心脏电压门控性钠通道)通道蛋白。方法设计并合成Nav1.5蛋白质的部分肽链作抗原免疫新西兰大耳白兔,制备抗体。经亲和层析提取纯化并生物素化,分别作为包被或检测抗体使用。建立检测人血浆Nav1.5通道蛋白的ELISA方法并优化条件。结果获得抗人血浆Nav1.5通道蛋白的抗体,并成功建立ELISA检测人血浆Nav1.5通道蛋白的方法。用该方法检测Brugada 1型患者血浆中Nav1.5钠通道蛋白的表达水平,与正常人的比较差异显著。结论建立高效特异检测人血浆Nav1.5通道蛋白的ELISA方法,为临床研究Nav1.5通道蛋白提供一个可行的方案。Objective To establish an efficient, specific and sensitive ELISA method for the detection of Nav1. 5 channel proteins in human plasma. Methods A new polypeptide of Nay1.5 protein was designed and synthesized as antigen to immunize New Zealand white rabbits. They were purified and biotinylated by affinity chromatography and used as coating or detecting anti- bodies respectively. Establish an ELISA method for detecting Navl. 5 channel protein in human plasma and optimize the condi- tions. Results Antibodies to human plasma Navl. 5 channel protein were obtained, and an ELISA method for detecting Navl. 5 channel protein in human plasma was successfully established. The expression level of Nav1.5 channel protein in plasma of patients with Brugada syndrome was detected by this method. Compared with normal people, the difference is significant （P 〈7 0.05）. Conclusion Establish an efficient and specific ELISA method for detecting Nav1.5 channel protein in human plasma, and to provide a feasible scheme for clinical study of Nav1.5 channel protein.

关 键 词：Navl.5通道蛋白 酶联免疫吸附试验 离子通道病 

分 类 号：R446.61[医药卫生—诊断学]