基于自制水培装置培养的花生芽苗白藜芦醇含量分析  

Analysis of resveratrol content in peanut bud and seedling cultivated by self-made hydroponic device

作　　者：林贤伟 刘展眉[1] 程杏安[1] 蒋旭红[1] 吴波[1] 叶静敏 

机构地区：[1]仲恺农业工程学院天然产物化学研究所,广东广州510225

出　　处：《广东农业科学》2017年第11期146-151,共6页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(21406274;21376281);广东省大学生科技创新培育专项基金攀登计划项目(K1178350111);国家级大学生创新训练项目(201711347012);广东省大学生创新训练项目(201711347055)

摘　　要：研究花生芽不同培养方式、不同培养天数、不同组织部位白藜芦醇含量的差异及其进一步培养成花生苗白藜芦醇含量的变化。运用水培和土培两种方式进行花生发芽,利用高效液相色谱仪(HPLC)检测分析不同发芽天数花生芽白藜芦醇含量,选出最优发芽时间,再利用自制水培装置将花生芽培养成花生苗,对其根、茎、叶白藜芦醇含量进行分析。结果表明:花生芽发芽后5 d,其白藜芦醇含量最高,土培和水培含量分别为117.6、42.27μg/g。自制水培装置培养的花生苗根系发达,其白藜芦醇含量明显高于花生芽含量,花生苗根部白藜芦醇的含量达143.4μg/g,远高于茎、叶的含量。装置制备成本为350.0元,制作材料简单,使用操作简单,可种植各种蔬菜及花卉,改变了传统果蔬主要由大田种植的格局。The difference of resveratrol content in peanut sprouts under different culture modes,culture days and different tissue locations,and the changes of resveratrol content in peanut seedlings were studied. In this paper,peanut germination was carried out by two ways :water culture and soil culture,HPLC was used to detect and analyze resveratrol content under different culture days in peanut bud in order to select the best germination time,then using the self-made hydroponic device to further develop peanut bud into peanut seedlings,whose contents of resveratrol in roots,stems and leaves were analyzed. The results indicate that :the highest content of resveratrol was found in peanut bud in 5 th day of germination,the total resveratrol contents in soil and water cultured peanut bud were 117.6,42.27μg/g respectively. The root system of peanut seedlings was developed by self-made hydrcponic device,the total resveratrol contents in root and stem of peanut seedlings were higher than that in stems and leaves,with 143.4μg/g. The device is manufactureed by simple material,its operation is simple,and its cost is 350.0 Yuan. A variety of vegetables and flowers can be planted on it,it changes the pattern of traditional fruits and vegetables planted mainly in fields.

关 键 词：自制水培装置 仲恺花826 花生芽 白藜芦醇 

分 类 号：S317[农业科学—作物栽培与耕作技术]