真核细胞表达人骨形态发生蛋白-7的研究进展  

Progress of Human Bone Morphogenetic Protein-7 in Eukaryotic Cells

作　　者：薛俊英[1,2] 付加芳[1,2] 王世立[2] 

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,山东济南250022 [2]山东省医药生物技术研究中心卫生部生物技术药物重点实验室,山东济南250062

出　　处：《济南大学学报（自然科学版）》2017年第5期459-465,共7页Journal of University of Jinan(Science and Technology)

基　　金：山东省自然科学基金培养基金项目(ZR2014CP024);济南大学自然科学基金青年项目(XKY1418);山东省医学科学院医药卫生科技创新工程项目(2016)

摘　　要：针对目前生物系统表达的人骨形态发生蛋白-7(h BMP-7)产量不能满足市场需求量、活性低、成本高的现状,对目前h BMP-7在酵母表达系统、昆虫、哺乳动物组织细胞的表达状况进行综述,分析其各自的优势和局限性,并对h BMP-7真核系统表达进行展望,指出h BMP-7是一种有重要价值的生长因子,具有强烈的骨诱导活性,临床上可用于治疗骨折、骨不愈合,未来的研究重点应是h BMP-7表达量的问题。Aiming at the status of human bone morphogenetie protein-7 （hBMP-7） expressed by biological systems being low production, low activity, and high cost, the currently used eukaryotic expression systems to express hBMP-7 were re- viewed, such as Pichia pastoris, insect, mammalian tissue cells. Their advantages and limitations for expressing hBMP- 7 and the latest progress were analyzed respectively. The expression of hBMP-7 eukaryotic system was prospected. As a kind of important growth factors having strong bone induction activity and can be clinically used in the treatment of frac- tures and bone healing, the future research emphasis for hBMP-7 was pointed to be the expression of hBMP-7.

关 键 词：人骨形态发生蛋白-7 毕赤酵母 哺乳动物细胞 人骨肉瘤细胞U2-OS 

分 类 号：Q816[生物学—生物工程]