马铃薯块茎休眠解除过程中NOX酶活性变化及其蛋白质结构分析  

Changes of NOX Enzyme Activity During Progression of Potato Tuber Dormancy Release and its Protein Structure Analysis

作　　者：闫好禄 姬祥卓 文义凯[1,2] 唐勋 张彩霞 司怀军[1,2] 张宁[1,2] 

机构地区：[1]甘肃省作物遗传改良与种质创新重点实验室,甘肃省干旱生境作物学省部共建国家重点实验室培育基地,兰州730070 [2]甘肃农业大学生命科学技术学院,兰州730070

出　　处：《分子植物育种》2017年第12期4830-4834,共5页Molecular Plant Breeding

基　　金：国家自然科学基金(31560413);甘肃农业大学“伏羲人才”计划项目(FXRC20130102)共同资助

摘　　要：本研究测定了马铃薯块茎休眠解除过程中NADPH氧化酶(NADPH oxidase,NOX)的酶活性,用q RT-PCR方法检测了马铃薯St NOXs基因在块茎休眠解除过程中的相对表达量,并利用生物信息学方法分析了St NOX3基因编码蛋白质的可能结构和功能。情况表明:马铃薯块茎休眠解除过程中NOX酶活性呈升-降-升-降的动态变化过程;q RT-PCR情况显示St NOX3基因的相对表达量与NOX酶活性呈相同的变化趋势;生物信息学分析表明该基因编码940个氨基酸,蛋白质相对分子量为106 004.79,等电点为8.76。研究情况为进一步阐明St NOX基因家族在马铃薯块茎休眠解除过程中的作用奠定了基础。In this experim ent, the enzyme activity of NADPH oxidase was assayed in the progression of potato tuber dormancy release, during which, the relative expression of St NOXs gene was also detected by q RT-PCR method. The possible structure and potential function of the protein that was encoded by St NOX3 gene was analyzed by bioinformatics method. It can be depicted that the enzyme activity of NADPH oxidase showed up-down-updown dynamic changes in the course of potato tuber dormancy release; q RT-PCR showed that the relative expression of St NOX3 gene had the same variation tendency with NOX enzyme activity. Bioinformatics analysis demonstrated that NOX gene encoded 940 amino acids, the molecular weight of the protein was 106 004.79 and the isoelectric point was 8.76. The study laid the fundation for further elucidation of the role of St NOX gene family in potato tuber dormancy release.

关 键 词：马铃薯 块茎休眠 NOX基因 蛋白质结构 

分 类 号：S532[农业科学—作物学]