木薯苹果酸脱氢酶结构与功能预测及分析  

Bioinformatics Analysis of MDH Structure and Function in Cassava(Manihot esculenta)

作　　者：刘畅 薛晶晶[2] 秦于玲[2] 陈松笔[2] 

机构地区：[1]黑龙江八一农垦大学农学院,大庆158308 [2]中国热带农业科学院热带作物品种资源研究所,农业部木薯种质资源保护与利用重点实验室,儋州571737

出　　处：《分子植物育种》2017年第12期4835-4841,共7页Molecular Plant Breeding

基　　金：NSFC-CGIAR国际合作重点项目(31361140366);国家科技支撑项目(2015BAD15B01);2012年海南省创新创业人才启动基金共同资助

摘　　要：木薯(Manihot esculenta Crantz)是世界重要的粮食作物,块根中富含淀粉,而苹果酸脱氢酶(MDH)在淀粉和糖代谢中具有重要作用。本研究采用生物信息学方法,对木薯MDH的其氨基酸序列、导肽、跨膜结构域、亲/疏水性、二级和三维结构及功能域进行分析及预测。结果表明,MDH在8种植物中的氨基酸组成成分基本一致,二级结构最大元件均为α-螺旋;MDH在大麦中为叶绿体基质蛋白,在小麦中为细胞质基质蛋白,木薯及其他作物中为线粒体基质蛋白;MDH在木薯中有2个功能域。以野生木薯近缘种W14(M.esculenta ssp.flabellifolia)块根为对照,利用q RT-PCR从转录水平对野生橡胶木薯(M.glaziovii)和栽培种SC205和SC8块根的MDH基因表达进行分析,发现MDH基因在橡胶木薯、SC205和SC8中均下调表达,这些结果对进一步验证木薯MDH的生物学功能提供重要依据。Cassava（Manihot esculenta Crantz） is one of the most important staple crops. It has rich starch in storage root. Malate dehydrogenase（MDH） is a key enzyme, which plays an important role in starch and sucrose metabolism.In this study, the composition of amino acids, leader peptides, transmembrane segments, hydropho-bicity or hydrophilicity, 2-D and 3-D structures and functional domains of MDH protein in cassava were analyzed and predicted by using online software. The results showed that amino acid sequences in 8 kinds of plants were similar,and α-helix was the largest component in 2-D structure. MDH was a chloroplast matrix protein in barley, and a cytoplasmic matrix protein in wheat, MDH in cassava and other plants was mitochondrial matrix protein with two functional domains. Compared with a cassava wild relatives M. esculenta ssp. flabellifolia（W14）, The expressions of wild cassava M. glaziovii, M. esculenta SC205 and SC8 in storage roots were down regulated at the transcriptional level. These results would be helpful of further verify the biological functions of MDH in cassava.

关 键 词：木薯 MDH 结构与功能 生物信息学 

分 类 号：S533[农业科学—作物学]